To showcase the research that has generated the greatest interest among our readers, the Journal of Developmental Biology (JDB, ISSN 2221-3759) presents a selection of its most viewed papers published in 2025. Covering topics ranging from stem cell biology, embryogenesis, fertility, regeneration, neurodevelopment, and developmental signaling to epigenetic regulation, placental and cardiovascular development, and organogenesis, these articles highlight the diversity of research published in JDB. Together, they demonstrate the journal's commitment to advancing our understanding of developmental processes and their implications for human health and disease.

“CRISPR/Cas9-Targeted Myostatin Deletion Improves the Myogenic Differentiation Parameters for Muscle-Derived Stem Cells in Mice”
by Mohamed I. Elashry, Victoria C. Schneider, Manuela Heimann, Sabine Wenisch and Stefan Arnhold
J. Dev. Biol. 2025, 13(1), 5; https://doi.org/10.3390/jdb13010005
Available online: https://www.mdpi.com/2221-3759/13/1/5

“Utilizing C. elegans Spermatogenesis and Fertilization Mutants as a Model for Human Disease”
by Sofia M. Perez, Helena S. Augustineli and Matthew R. Marcello
J. Dev. Biol. 2025, 13(1), 4; https://doi.org/10.3390/jdb13010004
Available online: https://www.mdpi.com/2221-3759/13/1/4

“Ribosome Incorporation Transdifferentiates Chick Primary Cells and Induces Their Proliferation by Secreting Growth Factors”
by Shota Inoue, Arif Istiaq, Anamika Datta, Mengxue Lu, Shintaro Nakayama, Kousei Takashi, Nobushige Nakajo, Shigehiko Tamura, Ikko Kawashima and Kunimasa Ohta
J. Dev. Biol. 2025, 13(2), 19; https://doi.org/10.3390/jdb13020019
Available online: https://www.mdpi.com/2221-3759/13/2/19

“Zebrafish Unga Is Required for Genomic Maintenance upon Genotoxic Stress and Male Fertility”
by Latifa Kazzazy, Flóra Huba, Bálint Lóránt Hausz, Dávid Mező, Viktória Perey-Simon, Bálint Jezsó, Abdulrahman Seddik, Zoran Marinović, Judit Tóth, Angéla Békési et al.
J. Dev. Biol. 2025, 13(3), 32; https://doi.org/10.3390/jdb13030032
Available online: https://www.mdpi.com/2221-3759/13/3/32

“HP1-Mediated Silencing of the Doublesex1 Gene for Female Determination in the Crustacean Daphnia magna”
by Junya Leim, Nikko Adhitama, Quang Dang Nong, Pijar Religia, Yasuhiko Kato and Hajime Watanabe
J. Dev. Biol. 2025, 13(3), 23; https://doi.org/10.3390/jdb13030023
Available online: https://www.mdpi.com/2221-3759/13/3/23

“Super-Enhancers in Placental Development and Diseases”
by Gracy X. Rosario, Samuel Brown, Subhradip Karmakar, Mohammad A. Karim Rumi and Nihar R. Nayak
J. Dev. Biol. 2025, 13(2), 11; https://doi.org/10.3390/jdb13020011
Available online: https://www.mdpi.com/2221-3759/13/2/11

“Signaling Pathways in Human Blastocyst Development: From Molecular Mechanisms to In Vitro Optimization”
by Yan Jiao, Jiapeng Liu, Congge Li, Yuexin Hu and Sanjun Zhao
J. Dev. Biol. 2025, 13(3), 33; https://doi.org/10.3390/jdb13030033
Available online: https://www.mdpi.com/2221-3759/13/3/33

“Defective Neural Stem and Progenitor Cell Proliferation in Neurodevelopmental Disorders”
by Aki Shigenaka, Eri Nitta, Tadashi Nakagawa, Makiko Nakagawa and Toru Hosoi
J. Dev. Biol. 2025, 13(4), 40; https://doi.org/10.3390/jdb13040040
Available online: https://www.mdpi.com/2221-3759/13/4/40

“Neuronal Populations Involved in Motor Function Show Prominent Expression of Sbno1 During Postnatal Brain Development”
by Sunjidmaa Zolzaya, Dai Ihara, Munkhsoyol Erkhembaatar, Shinsuke Ochiai, Ayaka Isa, Mariko Nishibe, Jean-Pierre Bellier, Takahiro Shimizu, Satoshi Kikkawa, Ryo Nitta et al.
J. Dev. Biol. 2025, 13(1), 3; https://doi.org/10.3390/jdb13010003
Available online: https://www.mdpi.com/2221-3759/13/1/3

“Origins of Aortic Coarctation: A Vascular Smooth Muscle Compartment Boundary Model”
by Christina L. Greene, Geoffrey Traeger, Akshay Venkatesh, David Han and Mark W. Majesky
J. Dev. Biol. 2025, 13(2), 13; https://doi.org/10.3390/jdb13020013
Available online: https://www.mdpi.com/2221-3759/13/2/13

“Wound-Induced Regeneration in Feather Follicles: A Stepwise Strategy to Regenerate Stem Cells”
by Ting-Xin Jiang, Ping Wu, Ang Li, Randall B. Widelitz and Cheng-Ming Chuong
J. Dev. Biol. 2025, 13(2), 10; https://doi.org/10.3390/jdb13020010
Available online: https://www.mdpi.com/2221-3759/13/2/10

“Profilin and Non-Canonical Wnt Signaling: Coordinating Cytoskeletal Dynamics from Development to Disease”
by Samira Alam, Danielle Duncan and Sharmin Hasan
J. Dev. Biol. 2025, 13(3), 31; https://doi.org/10.3390/jdb13030031
Available online: https://www.mdpi.com/2221-3759/13/3/31

“The Congenital Malformation of the Interatrial Septum—A Review of Its Development and Embryology with Clinical Implications”
by Rui Caetano Oliveira, Paula Martins, and Maria de Fátima Martins
J. Dev. Biol. 2025, 13(3), 28; https://doi.org/10.3390/jdb13030028
Available online: https://www.mdpi.com/2221-3759/13/3/28

“Pathophysiology and Management of Placenta Accreta Spectrum”
by Lana Shteynman, Genevieve Monanian, Gilberto Torres, Giancarlo Sabetta, Deborah M. Li, Zhaosheng Jin, Tiffany Angelo, Bahaa E. Daoud and Morgane Factor
J. Dev. Biol. 2025, 13(4), 45; https://doi.org/10.3390/jdb13040045
Available online: https://www.mdpi.com/2221-3759/13/4/45 

“GLP-1-Mediated Pregnancy and Neonatal Complications in Mice”
by Rajalakshmi Ramamoorthy, Arianna K. Carden, Hussain Hussain, Brian Z. Druyan, Ping Ping Chen, Rima Hajjar, Carmen Fernandez, Nila Elumalai, Amirah B. Rashed, Karen Young et al.
J. Dev. Biol. 2025, 13(3), 29; https://doi.org/10.3390/jdb13030029
Available online: https://www.mdpi.com/2221-3759/13/3/29

“In Vitro Embryo Culture Impacts Heart Mitochondria in Male Adolescent Sheep”
by Reza Amanollahi, Stacey L. Holman, Ashley S. Meakin, Monalisa Padhee, Kimberley J. Botting-Lawford, Song Zhang, Severence M. MacLaughlin, David O. Kleemann, Simon K. Walker, Jennifer M. Kelly et al.
J. Dev. Biol. 2025, 13(2), 17; https://doi.org/10.3390/jdb13020017
Available online: https://www.mdpi.com/2221-3759/13/2/17

“Varanid Teeth Asymmetry and Correlation to Body Size”
by Guy Sion and Domenic C. D’Amore
J. Dev. Biol. 2025, 13(1), 9; https://doi.org/10.3390/jdb13010009
Available online: https://www.mdpi.com/2221-3759/13/1/9

“Effects of Molybdenum Supplementation in the Form of Ammonium and Sodium Salts on Trophoblast Cell Physiology and Gene Expression In Vitro”
by Vladimira Foteva, Joshua J. Fisher, Yixue Qiao and Roger Smith
J. Dev. Biol. 2025, 13(1), 8; https://doi.org/10.3390/jdb13010008
Available online: https://www.mdpi.com/2221-3759/13/1/8

“Recapitulating Liver Embryology—Lessons to Be Learned for Liver Diseases”
by Rui Caetano Oliveira, Sandra Ferreira, Isabel Gonçalves and Maria Fátima Martins
J. Dev. Biol. 2025, 13(4), 39; https://doi.org/10.3390/jdb13040039
Available online: https://www.mdpi.com/2221-3759/13/4/39

“Probe Sequencing Analysis of Regenerating Lizard Tails Indicates Crosstalk Among Osteoclasts, Epidermal Cells, and Fibroblasts”
by Darian J. Gamble, Samantha Lopez, Melody Yazdi, Toni Castro-Torres and Thomas P. Lozito
J. Dev. Biol. 2025, 13(2), 15; https://doi.org/10.3390/jdb13020015
Available online: https://www.mdpi.com/2221-3759/13/2/15

“Cannabinoid Receptor 1 Regulates Zebrafish Renal Multiciliated Cell Development via cAMP Signaling”
by Thanh Khoa Nguyen, Sophia Baker, Julienne Angtuaco, Liana Arceri, Samuel Kaczor, Bram Fitzsimonds, Matthew R. Hawkins and Rebecca A. Wingert
J. Dev. Biol. 2025, 13(2), 10; https://doi.org/10.3390/jdb13020010
Available online: https://www.mdpi.com/2221-3759/13/2/20