Ammonia (NH
3) emissions from concentrated animal feeding operations (CAFOs) are recognized contributors to secondary fine particulate matter (PM
2.5) formation, yet empirically derived secondary PM
2.5 emission factors applicable to livestock operations remain limited. This study investigated NH
3-derived
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Ammonia (NH
3) emissions from concentrated animal feeding operations (CAFOs) are recognized contributors to secondary fine particulate matter (PM
2.5) formation, yet empirically derived secondary PM
2.5 emission factors applicable to livestock operations remain limited. This study investigated NH
3-derived secondary PM
2.5 formation under controlled laboratory conditions using a PTFE flow reactor in which NH
3 was reacted with sulfur dioxide (SO
2) across ammonia-rich NH
3:SO
2 ratios, with and without zero air. The resulting aerosols were characterized using gravimetric analysis, elemental analysis, Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM/EDS), and particle size distribution (PSD) measurements. The recovered particles were dominated by inorganic ammonium–sulfur species, with FTIR and elemental trends indicating sulfite-related intermediates under no-zero-air conditions and more oxidized ammonium–sulfur products under oxygenated conditions. Accounting for both filter-collected and wall-deposited particles, unit particulate emission factors normalized to ammonia input were derived. Size-based apportionment using PSD data indicated that approximately 76.6% of the recovered particulate mass was within the PM
2.5 size range. Scaling the experimentally derived unit emission factors using literature-based ammonia emission rates yielded an estimated secondary PM
2.5 emission factor of 0.351 ± 0.084 g PM
2.5 per animal head per day for cattle feedlots, corresponding to approximately 3–4% of reported total PM
2.5 emissions. Because the experimental system isolates NH
3–SO
2 interactions under idealized conditions and does not represent full atmospheric chemistry, the derived values should be interpreted as screening-level estimates of NH
3-derived secondary PM
2.5 formation potential intended to support comparative air quality assessments of CAFOs rather than direct predictions of ambient PM
2.5 concentrations.
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