This study investigated the effects of integrating biofloc with microalgae on growth performance and immune gene expression in red tilapia (
Oreochromis sp.). The experiment consisted of four treatments: C (Biofloc), T1 (
Chlorella vulgaris and
Nannochloropsis sp.; 1:1), T2 (Biofloc +
Chlorella
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This study investigated the effects of integrating biofloc with microalgae on growth performance and immune gene expression in red tilapia (
Oreochromis sp.). The experiment consisted of four treatments: C (Biofloc), T1 (
Chlorella vulgaris and
Nannochloropsis sp.; 1:1), T2 (Biofloc +
Chlorella vulgaris and
Nannochloropsis sp.; 1:1), T3 (Biofloc +
Chlorella vulgaris and
Nannochloropsis sp.; 2:1) in 500 L plastic tanks for 60 days. T2 and T3 exhibited the lowest ammonia and nitrite levels, respectively. T3 exhibited the highest chlorophyll a and chlorophyll b levels, while T2 showed the highest carotenoid content. T2 showed the highest weight gain (142 ± 0.7 g) and SGR (1.61 ± 0.02) and the lowest FCR (1.79 ± 0.009). T2 exhibited the highest gene expression levels in the intestine, with 7.8-fold upregulation of the cathepsin L (
ctsl) gene, 3-fold upregulation of toll-like receptor 7 (
tlr7), 6.7-fold upregulation of interleukin-1 b (
il-1b), 4.7-fold upregulation of tumor necrosis factor-alpha (
tnf-a), and 2.8-fold upregulation of metallothionein (
mt). In the head kidney, the
mt upregulation was highest in T3 (7.2-fold), while
tnf-a and
tlr7 upregulations were highest in T2 (5.9-fold and 5-fold, respectively). In the liver, the gene expressions were highest in T3, with 6.4-fold upregulation of
mt, 5-fold upregulation of
ctsl, 2.7-fold upregulation of
tlr7, 3-fold upregulation of
il-1b, and 5.4-fold upregulation of
tnf-a. These results suggest a synergistic effect of algae and bacteria on immune and antioxidative capacity in red tilapia.
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