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High-Throughput, Volume 7, Issue 2

2018 June - 10 articles

Cover Story: Multi-well plates and cell arrays enable microscopy-based screening in an arrayed format and, in contrast to pooled screening, enables high-content data collection for genetic and chemical libraries. Each of these formats possesses their own strengths and weaknesses, but reference comparisons between these platforms and their application rationale is lacking. We aim to fill this gap by comparing two RNAi-mediated fluorescence microscopy-based assays, namely EGF internalization and cell cycle progression, on both platforms. Quantitative analysis revealed that both platforms enabled the generation of data, with the appearance of the expected phenotypes being significantly distinct from the negative controls. Our data indicate that the choice of the screening platform primarily depends on the type of the cellular assay used to achieve maximum data quality and screen efficiency. View Paper here.
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Articles (10)

  • Review
  • Open Access
49 Citations
14,085 Views
13 Pages

Microfluidic Devices for Drug Assays

  • Clément Regnault,
  • Dharmendra S. Dheeman and
  • Axel Hochstetter

In this review, we give an overview of the current state of microfluidic-based high-throughput drug assays. In this highly interdisciplinary research field, various approaches have been applied to high-throughput drug screening, including microtiter...

  • Article
  • Open Access
2 Citations
4,319 Views
14 Pages

A Parallel Software Pipeline for DMET Microarray Genotyping Data Analysis

  • Giuseppe Agapito,
  • Pietro Hiram Guzzi and
  • Mario Cannataro

Personalized medicine is an aspect of the P4 medicine (predictive, preventive, personalized and participatory) based precisely on the customization of all medical characters of each subject. In personalized medicine, the development of medical treatm...

  • Review
  • Open Access
1 Citations
3,649 Views
9 Pages

Handling Complexity in Animal and Plant Science Research—From Single to Functional Traits: Are We There Yet?

  • Jessica Roberts,
  • Aoife Power,
  • Shaneel Chandra,
  • James Chapman and
  • Daniel Cozzolino

The current knowledge of the main factors governing livestock, crop and plant quality as well as yield in different species is incomplete. For example, this can be evidenced by the persistence of benchmark crop varieties for many decades in spite of...

  • Review
  • Open Access
16 Citations
6,437 Views
20 Pages

Biological nitrogen fixation gives legumes a pronounced growth advantage in nitrogen-deprived soils and is of considerable ecological and economic interest. In exchange for reduced atmospheric nitrogen, typically given to the plant in the form of ami...

  • Article
  • Open Access
1 Citations
4,011 Views
14 Pages

Computational Convolution of SELDI Data for the Diagnosis of Alzheimer’s Disease

  • Destiny E. O. Anyaiwe,
  • Gautam B. Singh,
  • George D. Wilson and
  • Timothy J. Geddes

Alzheimer’s disease is rapidly becoming an endemic for people over the age of 65. A vital path towards reversing this ominous trend is the building of reliable diagnostic devices for definite and early diagnoses in lieu of the longitudinal, usually i...

  • Communication
  • Open Access
4 Citations
6,157 Views
15 Pages

Comparison of Cell Arrays and Multi-Well Plates in Microscopy-Based Screening

  • Ann-Kristin Becker,
  • Holger Erfle,
  • Manuel Gunkel,
  • Nina Beil,
  • Lars Kaderali and
  • Vytaute Starkuviene

Multi-well plates and cell arrays enable microscopy-based screening assays in which many samples can be analysed in parallel. Each of the formats possesses its own strengths and weaknesses, but reference comparisons between these platforms and their...

  • Review
  • Open Access
19 Citations
7,348 Views
14 Pages

Exogenous reactive chemicals can impair cellular homeostasis and are often associated with the development of cancer. Significant progress has been achieved by studying the macromolecular interactions of chemicals that possess various electron-withdr...

  • Article
  • Open Access
18 Citations
7,909 Views
16 Pages

Functional magnetic resonance imaging (fMRI) is a non-invasive brain imaging technique, which has been regularly used for studying brain’s functional activities in the past few years. A very well-used measure for capturing functional associatio...

  • Article
  • Open Access
26 Citations
9,919 Views
12 Pages

Pre-transfusion bedside compatibility test is mandatory to check that the donor and the recipient present compatible groups before any transfusion is performed. Although blood typing devices are present on the market, they still suffer from various d...

  • Review
  • Open Access
117 Citations
13,114 Views
11 Pages

Metabolomics has made significant progress in multiple fronts in the last 18 months. This minireview aimed to give an overview of these advancements in the light of their contribution to targeted and untargeted metabolomics. New computational approac...

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High-Throughput - ISSN 2571-5135