Table of Contents
High-Throughput, Volume 7, Issue 2 (June 2018)
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Cover Story (view full-size image) Multi-well plates and cell arrays enable microscopy-based screening in an arrayed format and, in [...] Read more. Multi-well plates and cell arrays enable microscopy-based screening in an arrayed format and, in contrast to pooled screening, enables high-content data collection for genetic and chemical libraries. Each of these formats possesses their own strengths and weaknesses, but reference comparisons between these platforms and their application rationale is lacking. We aim to fill this gap by comparing two RNAi-mediated fluorescence microscopy-based assays, namely EGF internalization and cell cycle progression, on both platforms. Quantitative analysis revealed that both platforms enabled the generation of data, with the appearance of the expected phenotypes being significantly distinct from the negative controls. Our data indicate that the choice of the screening platform primarily depends on the type of the cellular assay used to achieve maximum data quality and screen efficiency. View Paper here.