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Open AccessCommunication
High-Throughput 2018, 7(2), 13;

Comparison of Cell Arrays and Multi-Well Plates in Microscopy-Based Screening

Institute of Bioinformatics, University Medicine Greifswald, 17475 Greifswald, Germany
BioQuant, Heidelberg University, 69120 Heidelberg, Germany
Institute of Biosciences, Vilnius University Life Sciences Center, LT-10257 Vilnius, Lithuania
Authors to whom correspondence should be addressed.
Received: 7 March 2018 / Revised: 8 May 2018 / Accepted: 9 May 2018 / Published: 15 May 2018
PDF [3274 KB, uploaded 15 May 2018]


Multi-well plates and cell arrays enable microscopy-based screening assays in which many samples can be analysed in parallel. Each of the formats possesses its own strengths and weaknesses, but reference comparisons between these platforms and their application rationale is lacking. We aim to fill this gap by comparing two RNA interference (RNAi)-mediated fluorescence microscopy-based assays, namely epidermal growth factor (EGF) internalization and cell cycle progression, on both platforms. Quantitative analysis revealed that both platforms enabled the generation of data with the appearance of the expected phenotypes significantly distinct from the negative controls. The measurements of cell cycle progression were less variable in multi-well plates. The result can largely be attributed to higher cell numbers resulting in less data variability when dealing with the assay generating phenotypic cell subpopulations. The EGF internalization assay with a uniform phenotype over nearly the whole cell population performed better on cell arrays than in multi-well plates. The result was achieved by scoring five times less cells on cell arrays than in multi-well plates, indicating the efficiency of the cell array format. Our data indicate that the choice of the screening platform primarily depends on the type of the cellular assay to achieve a maximum data quality and screen efficiency. View Full-Text
Keywords: cell arrays; cell-based screening; RNA interference cell arrays; cell-based screening; RNA interference

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Becker, A.-K.; Erfle, H.; Gunkel, M.; Beil, N.; Kaderali, L.; Starkuviene, V. Comparison of Cell Arrays and Multi-Well Plates in Microscopy-Based Screening. High-Throughput 2018, 7, 13.

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