Next Article in Journal
Computational Convolution of SELDI Data for the Diagnosis of Alzheimer’s Disease
Previous Article in Journal
Reactive Chemicals and Electrophilic Stress in Cancer: A Minireview
Article Menu
Issue 2 (June) cover image

Export Article

Open AccessCommunication
High-Throughput 2018, 7(2), 13; https://doi.org/10.3390/ht7020013

Comparison of Cell Arrays and Multi-Well Plates in Microscopy-Based Screening

1
Institute of Bioinformatics, University Medicine Greifswald, 17475 Greifswald, Germany
2
BioQuant, Heidelberg University, 69120 Heidelberg, Germany
3
Institute of Biosciences, Vilnius University Life Sciences Center, LT-10257 Vilnius, Lithuania
*
Authors to whom correspondence should be addressed.
Received: 7 March 2018 / Revised: 8 May 2018 / Accepted: 9 May 2018 / Published: 15 May 2018
Full-Text   |   PDF [3274 KB, uploaded 15 May 2018]   |  

Abstract

Multi-well plates and cell arrays enable microscopy-based screening assays in which many samples can be analysed in parallel. Each of the formats possesses its own strengths and weaknesses, but reference comparisons between these platforms and their application rationale is lacking. We aim to fill this gap by comparing two RNA interference (RNAi)-mediated fluorescence microscopy-based assays, namely epidermal growth factor (EGF) internalization and cell cycle progression, on both platforms. Quantitative analysis revealed that both platforms enabled the generation of data with the appearance of the expected phenotypes significantly distinct from the negative controls. The measurements of cell cycle progression were less variable in multi-well plates. The result can largely be attributed to higher cell numbers resulting in less data variability when dealing with the assay generating phenotypic cell subpopulations. The EGF internalization assay with a uniform phenotype over nearly the whole cell population performed better on cell arrays than in multi-well plates. The result was achieved by scoring five times less cells on cell arrays than in multi-well plates, indicating the efficiency of the cell array format. Our data indicate that the choice of the screening platform primarily depends on the type of the cellular assay to achieve a maximum data quality and screen efficiency. View Full-Text
Keywords: cell arrays; cell-based screening; RNA interference cell arrays; cell-based screening; RNA interference
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Becker, A.-K.; Erfle, H.; Gunkel, M.; Beil, N.; Kaderali, L.; Starkuviene, V. Comparison of Cell Arrays and Multi-Well Plates in Microscopy-Based Screening. High-Throughput 2018, 7, 13.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
High-Throughput EISSN 2571-5135 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top