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CRISPR-Cas9 Mediated Genome Editing in Bicyclus anynana Butterflies

Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore
Yale-NUS College, 10 College Avenue West, Singapore 138609, Singapore
Authors to whom correspondence should be addressed.
Methods Protoc. 2018, 1(2), 16;
Received: 26 April 2018 / Revised: 4 May 2018 / Accepted: 4 May 2018 / Published: 15 May 2018
(This article belongs to the Collection Gene Editing)
PDF [5340 KB, uploaded 17 May 2018]


CRISPR-Cas9 is revolutionizing the field of genome editing in non-model organisms. The robustness, ease of use, replicability and affordability of the technology has resulted in its widespread adoption among researchers. The African butterfly Bicyclus anynana is an emerging model lepidopteran species in the field of evo-devo, with a sequenced genome and amenable to germ line transformation. However, efficient genome editing tools to accelerate the pace of functional genetic research in this species have only recently become available with CRISPR-Cas9 technology. Here, we provide a detailed explanation of the CRISPR-Cas9 protocol we follow in the lab. The technique has been successfully implemented to knock-out genes associated with eyespot development and melanin pigmentation.
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Keywords: CRISPR-Cas9; sgRNA; Bicyclus anynana CRISPR-Cas9; sgRNA; Bicyclus anynana

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Banerjee, T.D.; Monteiro, A. CRISPR-Cas9 Mediated Genome Editing in Bicyclus anynana Butterflies. Methods Protoc. 2018, 1, 16.

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