Mining Genes Related to Single Fruit Weight of Peach (Prunus persica) Based on WGCNA and GSEA
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Phenotype Identification of Single Fruit Weight
2.3. RNA Extracting, Database Building and Data Filtering
2.4. Comparative Analysis of Reference Sequences and Differential Expression
2.5. Analysis of WGCNA
2.6. GSEA Analysis
2.7. Fluorescence Quantitative PCR Analysis
3. Results
3.1. Changes of Single Fruit Weight Traits during Peach Fruit Development
3.2. The Quality Evaluation of RNA-Seq Sequencing Data
3.3. Statistics of Differentially Expressed Genes
3.4. Screening Key Candidate Genes Based on WGCNA
3.5. Gene Set Enrichment Analysis GSEA
3.6. Screening of Key Genes for Single Fruit Weight by Three Methods
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bie, H.; Wang, H.; Wang, L.; Li, Y.; Fang, W.; Chen, C.; Wang, X.; Wu, J.; Cao, K. Mining Genes Related to Single Fruit Weight of Peach (Prunus persica) Based on WGCNA and GSEA. Horticulturae 2023, 9, 1335. https://doi.org/10.3390/horticulturae9121335
Bie H, Wang H, Wang L, Li Y, Fang W, Chen C, Wang X, Wu J, Cao K. Mining Genes Related to Single Fruit Weight of Peach (Prunus persica) Based on WGCNA and GSEA. Horticulturae. 2023; 9(12):1335. https://doi.org/10.3390/horticulturae9121335
Chicago/Turabian StyleBie, Hangling, Huimin Wang, Lirong Wang, Yong Li, Weichao Fang, Changwen Chen, Xinwei Wang, Jinlong Wu, and Ke Cao. 2023. "Mining Genes Related to Single Fruit Weight of Peach (Prunus persica) Based on WGCNA and GSEA" Horticulturae 9, no. 12: 1335. https://doi.org/10.3390/horticulturae9121335