Development of Cleaved Amplified Polymorphic Sequence Marker for Cap Color Identification in Pleurotus cornucopiae
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Materials
2.2. Experimental Methods
2.2.1. Primer Design and Synthesis
2.2.2. CAPS/dCAPS Marker Development and Restriction Enzyme Selection
2.2.3. PCR Amplification System and Program
2.2.4. Enzyme Digestion System and Electrophoresis Detection of the Digestion Products
3. Results
3.1. Primer Design and PCR Validation
3.2. CAPS Marker Development and Screening
3.3. Validation of CAPS/dCAPS Markers Co-Segregating with Cap Color
3.4. Validation of CAPS/dCAPS Markers in Other Dark-Colored Oyster mushrooms Varieties
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Marker | SNP | Primer Sequence (5′-3′) | Restriction Endonuclease |
---|---|---|---|
TYR-CAPS-1-1 | 1: −1590 bp T>C | F: GCCGAATAACGAACCAAAATGCGCG R: AGCAGTCGGAGCGTTCAAGTGGTTA | Hpy188 I (TCNGA) |
TYR-CAPS-1-2 | 1: −1590 bp T>C | F: GCCGAATAACGAACCAAAATGCGCG R: AGCAGTCGGAGCGTTCAAGTGGTTA | Nla IV (GGNNCC) |
TYR-dCAPS-2 | 2: 1038 bp T>C | F: TCTACATATATAAAGTTTAACTCTGACGACTGGATT R: ACTTTGAGCCGTCTTTGCATTCCTC | Nla III (CATG) |
TYR-CAPS-3-1 | 3: 1273 bp A>C | F: GTGCACGGATTGATACATTTGATA R: ATATCCGCCAGTCGTATTCATAA | Nla III (CATG) |
TYR-CAPS-3-2 | 3: 1273 bp A>C | F: GTGCACGGATTGATACATTTGATA R: ATATCCGCCAGTCGTATTCATAA | Mse I (TTAA) |
TYR-dCAPS-4 | 4: 1303bp G>C | F: ATCAACAACATCAACTACATATATTAATGTCGTCT R: GGGCCTCCATTAGGGTATCGTTCAT | Hpy188 I (TCNGA) |
Strain Number | Species |
---|---|
CCMSSC00328 | Pleurotus ostreatus |
CCMSSC00329 | Pleurotus ostreatus |
CCMSSC00363 | Pleurotus sp. |
CCMSSC00364 | Pleurotus sp. |
CCMSSC00499 | Pleurotus pulmonarius |
CCMSSC00630 | Pleurotus sp. |
CCMSSC03989 | Pleurotus sp. |
CCMSSC04195 | Pleurotus ostreatus |
CCMSSC00387 | Pleurotus sp. |
CCMSSC00322 | Pleurotus ostreatus |
CCMSSC04976 | Pleurotus sp. |
CCMSSC04977 | Pleurotus sp. |
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Yang, Y.; Zhang, Y.; Huang, C.; Chen, Q.; Gao, W. Development of Cleaved Amplified Polymorphic Sequence Marker for Cap Color Identification in Pleurotus cornucopiae. Horticulturae 2023, 9, 1238. https://doi.org/10.3390/horticulturae9111238
Yang Y, Zhang Y, Huang C, Chen Q, Gao W. Development of Cleaved Amplified Polymorphic Sequence Marker for Cap Color Identification in Pleurotus cornucopiae. Horticulturae. 2023; 9(11):1238. https://doi.org/10.3390/horticulturae9111238
Chicago/Turabian StyleYang, Yashu, Yan Zhang, Chenyang Huang, Qiang Chen, and Wei Gao. 2023. "Development of Cleaved Amplified Polymorphic Sequence Marker for Cap Color Identification in Pleurotus cornucopiae" Horticulturae 9, no. 11: 1238. https://doi.org/10.3390/horticulturae9111238
APA StyleYang, Y., Zhang, Y., Huang, C., Chen, Q., & Gao, W. (2023). Development of Cleaved Amplified Polymorphic Sequence Marker for Cap Color Identification in Pleurotus cornucopiae. Horticulturae, 9(11), 1238. https://doi.org/10.3390/horticulturae9111238