Next Article in Journal
Gene Expression Analysis in Cold Stress Conditions Reveals BBX20 and CLO as Potential Biomarkers for Cold Tolerance in Almond
Next Article in Special Issue
Use of Biotechnological Methods to Support the Production of New Peach Hybrids
Previous Article in Journal
Nutrient Deficiency Affects the Growth and Nitrate Concentration of Hydroponic Radish
Previous Article in Special Issue
Somatic Embryogenesis in Vitis for Genome Editing: Optimization of Protocols for Recalcitrant Genotypes
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Horticulturae
Volume 7
Issue 12
10.3390/horticulturae7120526
horticulturae-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Endophytic Bacteria in Banana In Vitro Cultures: Molecular Identification, Antibiotic Susceptibility, and Plant Survival

by
Antar Nasr El-Banna
1,2,
Mohammed Elsayed El-Mahrouk
3,
Yaser Hassan Dewir
4,*,
Mona Ali Farid
1,
Doaa Mahmoud Abou Elyazid
3 and
Heinz Martin Schumacher
5
1
Genetics Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt
2
Julius Kühn-Institut (JKI) Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Messeweg 11-12, 38104 Braunschweig, Germany
3
Department of Horticulture, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt
4
Plant Production Department, College of Food & Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia
5
DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7b, 38124 Braunschweig, Germany
*
Author to whom correspondence should be addressed.
Horticulturae 2021, 7(12), 526; https://doi.org/10.3390/horticulturae7120526
Submission received: 27 October 2021 / Revised: 21 November 2021 / Accepted: 25 November 2021 / Published: 28 November 2021
(This article belongs to the Collection Application of Tissue Culture to Horticulture)
Browse Figures
Versions Notes

Abstract

Microbial contamination is a common problem that causes significant losses in plant micropropagation systems. The present study reports on the identification and control of bacterial contaminants in banana in vitro cultures. Twelve isolates belonging to Bacillus pumilus (S2), Bacillus subtilis (R2 and M4), Geobacillus stearothermophilus (S1, S3, S4, P2, M3 and R3) and Paenibacillus spp. (P1, R1 and M2) were identified by sequencing of 16S rRNA, gyrA or gyrB genes. Antibiotic susceptibility testing was performed with the disk diffusion method on bacterial isolates using 36 antimicrobial agents. Some antibiotics, notably Ticarcillin, Penicillin, Ampicillin, Cefazolin and Imipenem, had a broader range of bactericidal activity than others did. When contaminated axillary shoot cultures of banana were treated with 100 or 200 mg·L−1 of ticarcillin, ampicillin or penicillin the bacteria were eliminated, but a reduction in shoot multiplication and growth was observed. These findings contribute to minimizing the losses in the commercial micropropagation of banana.
Keywords: 16S rDNA; chlorosis; micropropagation; microbial contamination; tissue browning 16S rDNA; chlorosis; micropropagation; microbial contamination; tissue browning

Share and Cite

MDPI and ACS Style

El-Banna, A.N.; El-Mahrouk, M.E.; Dewir, Y.H.; Farid, M.A.; Abou Elyazid, D.M.; Schumacher, H.M. Endophytic Bacteria in Banana In Vitro Cultures: Molecular Identification, Antibiotic Susceptibility, and Plant Survival. Horticulturae 2021, 7, 526. https://doi.org/10.3390/horticulturae7120526

AMA Style

El-Banna AN, El-Mahrouk ME, Dewir YH, Farid MA, Abou Elyazid DM, Schumacher HM. Endophytic Bacteria in Banana In Vitro Cultures: Molecular Identification, Antibiotic Susceptibility, and Plant Survival. Horticulturae. 2021; 7(12):526. https://doi.org/10.3390/horticulturae7120526

Chicago/Turabian Style

El-Banna, Antar Nasr, Mohammed Elsayed El-Mahrouk, Yaser Hassan Dewir, Mona Ali Farid, Doaa Mahmoud Abou Elyazid, and Heinz Martin Schumacher. 2021. "Endophytic Bacteria in Banana In Vitro Cultures: Molecular Identification, Antibiotic Susceptibility, and Plant Survival" Horticulturae 7, no. 12: 526. https://doi.org/10.3390/horticulturae7120526

APA Style

El-Banna, A. N., El-Mahrouk, M. E., Dewir, Y. H., Farid, M. A., Abou Elyazid, D. M., & Schumacher, H. M. (2021). Endophytic Bacteria in Banana In Vitro Cultures: Molecular Identification, Antibiotic Susceptibility, and Plant Survival. Horticulturae, 7(12), 526. https://doi.org/10.3390/horticulturae7120526

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop