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Volume 9
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Article

Increased Flux of Lipid Metabolism Enhances Bioethanol Fermentability and Inhibitor Tolerance of Xylose-Utilizing Zymomonas mobilis

by
Junyi Hu
1,
William Wang
2,
Feifei Zhang
1,
Xuequan Jiang
1,
Lida Peng
1,
Yichao Fang
1 and
Haoyong Wang
1,*
1
Hubei Key Laboratory of Industrial Microbiology, Key Laboratory of Fermentation Engineering (Ministry of Education), National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei University of Technology, Wuhan 430068, China
2
Grinnell College, 1115 8th Avenue, Grinnell, IA 50112, USA
*
Author to whom correspondence should be addressed.
Fermentation 2023, 9(6), 569; https://doi.org/10.3390/fermentation9060569
Submission received: 25 April 2023 / Revised: 26 May 2023 / Accepted: 12 June 2023 / Published: 16 June 2023
(This article belongs to the Topic Waste-to-Energy)
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Abstract

The microbial production of fuel ethanol is an attractive and sustainable biotechnological approach. This study presents a metabolic engineering strategy of Zymomonas mobilis aimed at coproducing bioethanol and fatty acids. The increased flux of fatty acids stabilizes the cell membrane and thus counteracts the progressively higher ethanol toxicity. In a glucose medium, the highest ethanol titer achieved was 146.7 g/kg of broth, surpassing the wild-type Z. mobilis CP4 and angel yeast by 30% and 45%, respectively. The recombinant strain exhibited a total fatty acid titer of 0.4 g/L from 230 g/L total sugar solution (5 L bioreactor), representing a 12-fold increase compared to the wild-type Z. mobilis CP4. Furthermore, when using a 4:2:1 mixture of glucose: xylose: mannose (w/v), an ethanol concentration of 142.8 g/kg of broth was attained, only 2.66% lower than that of the glucose-only medium. These findings highlight the enormous potential of this genetically engineered strain for the sustainable production of ethanol and fatty acids from lignocellulosic renewable carbon sources.
Keywords: metabolic engineering; Zymomonas mobilis; bioethanol; fatty acids metabolic engineering; Zymomonas mobilis; bioethanol; fatty acids
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MDPI and ACS Style

Hu, J.; Wang, W.; Zhang, F.; Jiang, X.; Peng, L.; Fang, Y.; Wang, H. Increased Flux of Lipid Metabolism Enhances Bioethanol Fermentability and Inhibitor Tolerance of Xylose-Utilizing Zymomonas mobilis. Fermentation 2023, 9, 569. https://doi.org/10.3390/fermentation9060569

AMA Style

Hu J, Wang W, Zhang F, Jiang X, Peng L, Fang Y, Wang H. Increased Flux of Lipid Metabolism Enhances Bioethanol Fermentability and Inhibitor Tolerance of Xylose-Utilizing Zymomonas mobilis. Fermentation. 2023; 9(6):569. https://doi.org/10.3390/fermentation9060569

Chicago/Turabian Style

Hu, Junyi, William Wang, Feifei Zhang, Xuequan Jiang, Lida Peng, Yichao Fang, and Haoyong Wang. 2023. "Increased Flux of Lipid Metabolism Enhances Bioethanol Fermentability and Inhibitor Tolerance of Xylose-Utilizing Zymomonas mobilis" Fermentation 9, no. 6: 569. https://doi.org/10.3390/fermentation9060569

APA Style

Hu, J., Wang, W., Zhang, F., Jiang, X., Peng, L., Fang, Y., & Wang, H. (2023). Increased Flux of Lipid Metabolism Enhances Bioethanol Fermentability and Inhibitor Tolerance of Xylose-Utilizing Zymomonas mobilis. Fermentation, 9(6), 569. https://doi.org/10.3390/fermentation9060569

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