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Open AccessFeature PaperArticle

Impact of Organic Acids Supplementation to Hardwood Spent Sulfite Liquor as Substrate for the Selection of Polyhydroxyalkanoates-Producing Organisms

1
Departamento de Química/CICECO, Universidade de Aveiro, Campus Universitário de Santiago, P-3810-193 Aveiro, Portugal
2
Laboratório Associado para a Química Verde, Rede de Química e Tecnologia (LAQV, REQUIMTE), Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, P-2829-516 Caparica, Portugal
3
Water Research Institute-Consiglio Nazionale della Ricerche (CNR), Via Salaria Km 29,300, 00015 Monterotondo, Italy
*
Author to whom correspondence should be addressed.
Fermentation 2018, 4(3), 58; https://doi.org/10.3390/fermentation4030058
Received: 12 July 2018 / Revised: 18 July 2018 / Accepted: 18 July 2018 / Published: 24 July 2018
(This article belongs to the Special Issue Microbial Production of Added-value Products from Renewable Resources)
The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ± 2.7%), Alphaproteobacteria (13.6 ± 1.3%) and Gammaproteobacteria (2.40 ± 1.1%) and Bacteroidetes (9.20 ± 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus. View Full-Text
Keywords: polyhydroxyalkanoates; mixed microbial cultures; hardwood spent sulfite liquor; short-chain organic acids; Fluorescence in situ Hybridization (FISH) polyhydroxyalkanoates; mixed microbial cultures; hardwood spent sulfite liquor; short-chain organic acids; Fluorescence in situ Hybridization (FISH)
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MDPI and ACS Style

Queirós, D.; Rangel, C.; Costa Lemos, P.; Rossetti, S.; Seuanes Serafim, L. Impact of Organic Acids Supplementation to Hardwood Spent Sulfite Liquor as Substrate for the Selection of Polyhydroxyalkanoates-Producing Organisms. Fermentation 2018, 4, 58.

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