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Open AccessArticle

Two-Stage Aeration Fermentation Strategy to Improve Bioethanol Production by Scheffersomyces stipitis

CICECO—Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal
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Fermentation 2018, 4(4), 97; https://doi.org/10.3390/fermentation4040097
Received: 28 October 2018 / Revised: 15 November 2018 / Accepted: 16 November 2018 / Published: 20 November 2018
(This article belongs to the Special Issue Microbial Production of Added-value Products from Renewable Resources)
Hardwood spent sulfite liquor (HSSL) is a by-product from pulp industry with a high concentration of pentose sugars, besides some hexoses suitable for bioethanol production by Scheffersomyces stipitis. The establishment of optimal aeration process conditions that results in specific microaerophilic conditions required by S. stipitis is the main challenge for ethanol production. The present study aimed to improve the ethanol production from HSSL by S. stipitis through a two-stage aeration fermentation. Experiments with controlled dissolved oxygen tension (DOT) in the first stage and oxygen restriction in the second stage were carried out. The best results were obtained with DOT control at 50% in the first stage, where the increase of oxygen availability provided faster growth and higher biomass yield, and no oxygen supply with an agitation rate of 250 rpm, in the second stage allowed a successful induction of ethanol production. Fermentation using 60% of HSSL (v/v) as substrate for S. stipitis provided a maximum specific growth rate of 0.07 h−1, an ethanol productivity of 0.04 g L h−1 and an ethanol yield of 0.39 g g−1, respectively. This work showed a successful two-stage aeration strategy as a promising aeration alternative for bioethanol production from HSSL by S. stipitis. View Full-Text
Keywords: bioethanol; hardwood spent sulfite liquor; Scheffersomyces stipitis; two-stage aeration strategy; dissolved oxygen tension bioethanol; hardwood spent sulfite liquor; Scheffersomyces stipitis; two-stage aeration strategy; dissolved oxygen tension
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Henriques, T.M.; Pereira, S.R.; Serafim, L.S.; Xavier, A.M.R.B. Two-Stage Aeration Fermentation Strategy to Improve Bioethanol Production by Scheffersomyces stipitis. Fermentation 2018, 4, 97.

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