Next Article in Journal
The Diversity of Yeasts in Beekeeping Environments and the Selection of a Culture Starter for the Development of a Mead
Previous Article in Journal
The Impact of Varying Levels of Laurus nobilis Leaves as a Sustainable Feed Additive on Ruminal Fermentation: In Vitro Gas Production, Methane and Carbon Dioxide Emissions, and Ruminal Degradability of a Conventional Diet for Ruminants
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Fermentation
Volume 10
Issue 8
10.3390/fermentation10080388
fermentation-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Optimization of Microbial Glycogen Production by Saccharomyces cerevisiae CEY1

by
Hyungseok Choi
1,
In-Seok Yeo
2,
Godfrey Mwiti
1,
Toan Nguyen Song Dinh
1,
Hyein Kang
1,
Chang Sup Kim
3 and
Jaehan Kim
1,*
1
Department of Food and Nutrition, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea
2
Graduate School of Analytical Science and Technology, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea
3
Department of Chemical & Biological Engineering, Hanbat National University, 125 Dongseo-Daero, Yuseong-gu, Daejeon 34158, Republic of Korea
*
Author to whom correspondence should be addressed.
Fermentation 2024, 10(8), 388; https://doi.org/10.3390/fermentation10080388
Submission received: 10 June 2024 / Revised: 20 July 2024 / Accepted: 26 July 2024 / Published: 29 July 2024
(This article belongs to the Section Industrial Fermentation)
Browse Figures
Versions Notes

Abstract

Glycogen is a highly branched polyglucan utilized as a carbohydrate reserve in major living systems. Industrially, it is used as a prebiotic and in the nanoencapsulation of drugs and nutraceuticals. In this study, optimal fermentation conditions enabling the highest glycogen accumulation in Saccharomyces cerevisiae were experimentally evaluated for possible mass production. Production efficiency was assessed by comparing specific growth rates, specific glycogen production rates, and glycogen yields under each condition. The results demonstrated that fermentation at 30 °C with an aeration rate of 3 vvm using a medium containing 120 g/L glucose without ethanol was optimal for robust cell growth and maximum glycogen yield. Additionally, a rich medium outperformed a minimally defined medium, and a single sugar carbon source, as opposed to mixed sugars, resulted in significantly higher cell growth and glycogen yields (p < 0.05). The optimized fermentation parameters enabled a glycogen production rate of up to 0.232 ± 0.012 g-glycogen/g-cell/h and a glycogen yield of 0.603 ± 0.006 g-glycogen/g-glucose. These results provide meaningful information for future studies and/or large-scale glycogen production using S. cerevisiae.
Keywords: fermentation; glycogen; optimization; Saccharomyces cerevisiae; polyglucans fermentation; glycogen; optimization; Saccharomyces cerevisiae; polyglucans

Share and Cite

MDPI and ACS Style

Choi, H.; Yeo, I.-S.; Mwiti, G.; Dinh, T.N.S.; Kang, H.; Kim, C.S.; Kim, J. Optimization of Microbial Glycogen Production by Saccharomyces cerevisiae CEY1. Fermentation 2024, 10, 388. https://doi.org/10.3390/fermentation10080388

AMA Style

Choi H, Yeo I-S, Mwiti G, Dinh TNS, Kang H, Kim CS, Kim J. Optimization of Microbial Glycogen Production by Saccharomyces cerevisiae CEY1. Fermentation. 2024; 10(8):388. https://doi.org/10.3390/fermentation10080388

Chicago/Turabian Style

Choi, Hyungseok, In-Seok Yeo, Godfrey Mwiti, Toan Nguyen Song Dinh, Hyein Kang, Chang Sup Kim, and Jaehan Kim. 2024. "Optimization of Microbial Glycogen Production by Saccharomyces cerevisiae CEY1" Fermentation 10, no. 8: 388. https://doi.org/10.3390/fermentation10080388

APA Style

Choi, H., Yeo, I.-S., Mwiti, G., Dinh, T. N. S., Kang, H., Kim, C. S., & Kim, J. (2024). Optimization of Microbial Glycogen Production by Saccharomyces cerevisiae CEY1. Fermentation, 10(8), 388. https://doi.org/10.3390/fermentation10080388

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop