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Article

Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species

by
Rebeca Vázquez-Avendaño
1,
José Benjamín Rodríguez-Haas
1,
Hugo Velázquez-Delgado
1,
Greta Hanako Rosas-Saito
2,
Eric Edmundo Hernández-Domínguez
3,4,* and
Diana Sánchez-Rangel
1,4,*
1
Laboratorios de Biología Molecular y Fitopatología, Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa 91070, Veracruz, Mexico
2
Laboratorio de Microscopía Avanzada, Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa 91070, Veracruz, Mexico
3
Laboratorio de Proteómica, Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa 91070, Veracruz, Mexico
4
Catedrático CONACyT en la Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa 91070, Veracruz, Mexico
*
Authors to whom correspondence should be addressed.
J. Fungi 2021, 7(12), 1027; https://doi.org/10.3390/jof7121027
Submission received: 17 September 2021 / Revised: 26 November 2021 / Accepted: 26 November 2021 / Published: 30 November 2021
(This article belongs to the Special Issue Plant and Fungal Interactions)
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Abstract

Neofusicoccum parvum belongs to the Botryosphaeriaceae family, which contains endophytes and pathogens of woody plants. In this study, we isolated 11 strains from diseased tissue of Liquidambar styraciflua. Testing with Koch’s postulates—followed by a molecular approach—revealed that N. parvum was the most pathogenic strain. We established an in vitro pathosystem (L. styraciflua foliar tissue–N. parvum) in order to characterize the infection process during the first 16 days. New CysRPs were identified for both organisms using public transcriptomic and genomic databases, while mRNA expression of CysRPs was analyzed by RT-qPCR. The results showed that N. parvum caused disease symptoms after 24 h that intensified over time. Through in silico analysis, 5 CysRPs were identified for each organism, revealing that all of the proteins are potentially secreted and novel, including two of N. parvum proteins containing the CFEM domain. Interestingly, the levels of the CysRPs mRNAs change during the interaction. This study reports N. parvum as a pathogen of L. styraciflua for the first time and highlights the potential involvement of CysRPs in both organisms during this interaction.
Keywords: Neofusicoccum parvum; Liquidambar styraciflua; cysteine-rich proteins; CFEM domain-containing protein; plant fungal infection Neofusicoccum parvum; Liquidambar styraciflua; cysteine-rich proteins; CFEM domain-containing protein; plant fungal infection
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MDPI and ACS Style

Vázquez-Avendaño, R.; Rodríguez-Haas, J.B.; Velázquez-Delgado, H.; Rosas-Saito, G.H.; Hernández-Domínguez, E.E.; Sánchez-Rangel, D. Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species. J. Fungi 2021, 7, 1027. https://doi.org/10.3390/jof7121027

AMA Style

Vázquez-Avendaño R, Rodríguez-Haas JB, Velázquez-Delgado H, Rosas-Saito GH, Hernández-Domínguez EE, Sánchez-Rangel D. Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species. Journal of Fungi. 2021; 7(12):1027. https://doi.org/10.3390/jof7121027

Chicago/Turabian Style

Vázquez-Avendaño, Rebeca, José Benjamín Rodríguez-Haas, Hugo Velázquez-Delgado, Greta Hanako Rosas-Saito, Eric Edmundo Hernández-Domínguez, and Diana Sánchez-Rangel. 2021. "Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species" Journal of Fungi 7, no. 12: 1027. https://doi.org/10.3390/jof7121027

APA Style

Vázquez-Avendaño, R., Rodríguez-Haas, J. B., Velázquez-Delgado, H., Rosas-Saito, G. H., Hernández-Domínguez, E. E., & Sánchez-Rangel, D. (2021). Insights of the Neofusicoccum parvum–Liquidambar styraciflua Interaction and Identification of New Cysteine-Rich Proteins in Both Species. Journal of Fungi, 7(12), 1027. https://doi.org/10.3390/jof7121027

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