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Vet. Sci. 2018, 5(4), 89; https://doi.org/10.3390/vetsci5040089

In Vitro Anthelminthic Efficacy of Hypoestes forskaolii (Vahl) R.Br (Acanthaceae) Extracts on Gastrointestinal Nematodes of Sheep

1
Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, 80137 Naples, Italy
2
Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy
3
Department of Pharmacognosy, Faculty of Pharmacy, Umm Al-Qura University, 21955 Makkah, Saudi Arabia
*
Author to whom correspondence should be addressed.
Received: 20 July 2018 / Revised: 18 September 2018 / Accepted: 10 October 2018 / Published: 16 October 2018
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Abstract

The anthelminthic efficacy of the crude extracts of Hypoestes forskaolii (Vahl) R.Br (Acanthaceae) against gastrointestinal nematodes (GIN) in sheep was investigated using the in vitro egg hatch inhibition assay. Faecal samples were collected from sheep with naturally occurring infection of GIN (Trichostrongylus spp., Chabertia ovina, Cooperia spp., Haemonchus contortus and Teladorsagia spp.). Crude leaf extracts of H. forskaolii was obtained using increasing polarity solvents: n-hexane, chloroform, chloroform:methanol 9:1, methanol. Thiabendazole (0.2 µg/mL and 0.5 µg/mL) was used as a positive control and untreated GIN eggs in deionised water served as the negative control. All the extracts exhibited a weak ovicidal activity against GIN (less than 50% of egg hatch). Noteworthy, the n-hexane extract showed a percentage of inhibition of egg hatching greater than other extracts inhibiting the 30.8% at the concentration of 1 mg/mL showing a dose-dependent effect on nematode eggs hatching. Further studies are needed to investigate the effects of extracts used and to evaluate the ovicidal effects of other extracts of H. forskaolii. View Full-Text
Keywords: sheep; gastrointestinal nematodes; plant extracts; egg hatching assay; anthelminthic efficacy sheep; gastrointestinal nematodes; plant extracts; egg hatching assay; anthelminthic efficacy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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D’Ambola, M.; Bosco, A.; Ariano, A.; Rinaldi, L.; Bader, A.; Amadesi, A.; Cringoli, G.; Severino, L. In Vitro Anthelminthic Efficacy of Hypoestes forskaolii (Vahl) R.Br (Acanthaceae) Extracts on Gastrointestinal Nematodes of Sheep. Vet. Sci. 2018, 5, 89.

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