Comparative Serum Proteome Analysis Indicates a Negative Correlation between a Higher Immune Level and Feed Efficiency in Pigs
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Blood Collection
2.2. Protein Labeling and LC-MS/MS
2.3. Database Search and Bioinformatics
2.4. Bioinformatics Analyses
2.5. PRM-MS Analysis
3. Results
3.1. Basic Statistics of Porcine Performance and FE
3.2. Proteomic Differences between the HFE and LFE Groups
3.3. GO Annotation and KEGG Enrichment of DEPs
3.4. PPIs Network Construction and Analysis
3.5. DEPs Validated by PRM
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | LFE (Mean ± SD) | HFE (Mean ±SD) | p-Value a |
---|---|---|---|
N | 12 | 12 | |
RFI (kg) | 3.14 ± 0.95 | −2.69 ± 0.83 | <0.001 *** |
FCR | 2.88 ± 0.19 | 2.20 ± 0.14 | <0.001 *** |
ADFI (kg/d) | 2.90 ± 0.20 | 2.31 ± 0.18 | <0.001 *** |
ADG (kg/d) | 1.01 ± 0.10 | 1.03 ± 0.07 | 0.56 |
100 kgBF (mm) | 13.05 ± 3.35 | 12.46 ± 2.41 | 0.62 |
On-BW (kg) | 41.67 ± 5.46 | 40.33 ± 6.70 | 0.46 |
Off-BW (kg) | 112.37 ± 8.66 | 110.57 ± 5.92 | 0.56 |
Metamid-BW | 57.76 ± 4.05 | 56.59 ± 2.99 | 0.43 |
Protein Accession | Protein Gene | Fold Change (HFE/LFE) in TMT | p-Value in TMT | Fold Change (HFE/LFE) in PRM | p-Value in PRM |
---|---|---|---|---|---|
F1SFI7 | AHSG | 2.52 | 1.50 × 10−2 | 2.23 | 1.13 × 10−1 |
F1SJW8 | SERPING1 | 1.65 | 1.30 × 10−2 | 1.26 | 1.24 × 10−1 |
F1S133 | CFI | 2.23 | 3.10 × 10−2 | 2.17 | 1.50 × 10−1 |
Q29594 | CKB | 0.83 | 3.50 × 10−3 | 0.39 | 4.48 × 10−3 |
T1UNN8 | ANGPTL8 | 0.75 | 4.60 × 10−3 | 0.37 | 3.53 × 10−3 |
G9F6X8 | P4HB | 0.78 | 2.30 × 10−2 | 0.43 | 2.60 × 10−3 |
A0A287BAZ6 | PRDX2 | 0.78 | 8.10 × 10−3 | 0.44 | 2.93 × 10−3 |
A0A287BKS2 | PTX3 | 0.77 | 9.60 × 10−3 | 0.37 | 4.27× 10−2 |
I3LGB2 | PCSK9 | 0.82 | 1.80 × 10−2 | 0.49 | 2.44 × 10−4 |
F1RHA9 | LECT2 | 0.78 | 2.20 × 10−2 | 0.50 | 1.26 × 10−2 |
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Zhu, S.; Si, J.; Zhang, H.; Qi, W.; Zhang, G.; Yan, X.; Huang, Y.; Zhao, M.; Guo, Y.; Liang, J.; et al. Comparative Serum Proteome Analysis Indicates a Negative Correlation between a Higher Immune Level and Feed Efficiency in Pigs. Vet. Sci. 2023, 10, 338. https://doi.org/10.3390/vetsci10050338
Zhu S, Si J, Zhang H, Qi W, Zhang G, Yan X, Huang Y, Zhao M, Guo Y, Liang J, et al. Comparative Serum Proteome Analysis Indicates a Negative Correlation between a Higher Immune Level and Feed Efficiency in Pigs. Veterinary Sciences. 2023; 10(5):338. https://doi.org/10.3390/vetsci10050338
Chicago/Turabian StyleZhu, Siran, Jinglei Si, Huijie Zhang, Wenjing Qi, Guangjie Zhang, Xueyu Yan, Ye Huang, Mingwei Zhao, Yafen Guo, Jing Liang, and et al. 2023. "Comparative Serum Proteome Analysis Indicates a Negative Correlation between a Higher Immune Level and Feed Efficiency in Pigs" Veterinary Sciences 10, no. 5: 338. https://doi.org/10.3390/vetsci10050338