Transcriptomic and Metabolomic Changes Reveal the Immunomodulatory Function of Casein Phosphopeptide-Selenium Chelate in Beagle Dogs
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Experimental Design
2.2. Hematology Routine and Serum Cytokines Concentration Detection
2.3. RNA-Seq and Data Analysis
2.3.1. RNA Library Construction and Sequencing
2.3.2. Quality Control and Differentially Expressed Genes (DEGs) Identification
2.3.3. Functional Enrichment Analysis of DEGs
2.4. Screening for Immune-Related Genes
2.5. Protein–Protein Interaction (PPI) Network Construction and Hub Genes Analysis of DEGs
2.6. Untargeted Metabolomics and Data Analysis
2.6.1. Metabolites Extraction
2.6.2. UHPLC-MS/MS Analysis
2.6.3. Data Processing and Metabolite Identification
2.6.4. Data Analysis
2.7. Combined Analysis of RNA-Seq and Metabolome Data
2.8. Total RNA Extraction, cDNA Synthesis and RT-qPCR
2.9. Statistical Analysis
3. Results
3.1. Evaluation of Immune-Related Parameters
3.2. Summary of RNA-Seq Data
3.3. DEGs and Functional Enrichment Analysis
3.4. Immune-Related Genes and Hub Genes Analysis of DEGs
3.5. Analysis of DEGs Related to Cytokine–Cytokine Receptor Interaction Signaling Pathway and TCR Signaling Pathway
3.6. Untargeted Metabolomics and DEMs Analyses
3.7. Combined Analysis of Transcriptomics and Metabolomics
3.8. RT-qPCR Validation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, W.; Xu, L.; Cao, Y.; Liu, G.; Lin, Q.; Mao, X. Transcriptomic and Metabolomic Changes Reveal the Immunomodulatory Function of Casein Phosphopeptide-Selenium Chelate in Beagle Dogs. Vet. Sci. 2023, 10, 345. https://doi.org/10.3390/vetsci10050345
Wang W, Xu L, Cao Y, Liu G, Lin Q, Mao X. Transcriptomic and Metabolomic Changes Reveal the Immunomodulatory Function of Casein Phosphopeptide-Selenium Chelate in Beagle Dogs. Veterinary Sciences. 2023; 10(5):345. https://doi.org/10.3390/vetsci10050345
Chicago/Turabian StyleWang, Wencan, Ling Xu, Yong Cao, Guo Liu, Qianru Lin, and Xin Mao. 2023. "Transcriptomic and Metabolomic Changes Reveal the Immunomodulatory Function of Casein Phosphopeptide-Selenium Chelate in Beagle Dogs" Veterinary Sciences 10, no. 5: 345. https://doi.org/10.3390/vetsci10050345