Regeneration Process of an Autologous Tissue-Engineered Trachea (aTET) in a Rat Patch Tracheoplasty Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Graft Preparation
2.2. Tracheoplasty Procedure
2.3. Evaluations of the Explanted Graft
2.4. Ethics
3. Results
3.1. Survival Rate
3.2. Gross Appearance of the Graft
3.3. Histological Examinations
- < Hematoxylin and Eosin and Masson’s Trichrome staining >
- < Immunofluorescence staining for the CD68 and alpha SMA antibodies >
- < Alcian Blue staining and Immunofluorescence staining for the alpha Tubulin >
- < Von Kossa staining >
- < Summary of histological findings in each period >
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Iwasaki, S.; Deguchi, K.; Iwai, R.; Nakayama, Y.; Okuyama, H. Regeneration Process of an Autologous Tissue-Engineered Trachea (aTET) in a Rat Patch Tracheoplasty Model. Bioengineering 2024, 11, 243. https://doi.org/10.3390/bioengineering11030243
Iwasaki S, Deguchi K, Iwai R, Nakayama Y, Okuyama H. Regeneration Process of an Autologous Tissue-Engineered Trachea (aTET) in a Rat Patch Tracheoplasty Model. Bioengineering. 2024; 11(3):243. https://doi.org/10.3390/bioengineering11030243
Chicago/Turabian StyleIwasaki, Shun, Koichi Deguchi, Ryosuke Iwai, Yasuhide Nakayama, and Hiroomi Okuyama. 2024. "Regeneration Process of an Autologous Tissue-Engineered Trachea (aTET) in a Rat Patch Tracheoplasty Model" Bioengineering 11, no. 3: 243. https://doi.org/10.3390/bioengineering11030243