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Article

Effects of 3-Month Exposure to E-Cigarette Aerosols on Glutamatergic Receptors and Transporters in Mesolimbic Brain Regions of Female C57BL/6 Mice

1
Department of Pharmacology and Experimental Therapeutics, College of Pharmacy and Pharmaceutical Sciences, the University of Toledo, Toledo, OH 43614, USA
2
Pulmonary and Critical Care Section, VA San Diego Healthcare System, 3350 La Jolla Village Dr, MC 111J, San Diego, CA 92161, USA
3
Department of Medicine, Division of Pulmonary and Critical Care, University of California at San Diego (UCSD), La Jolla, CA 92093, USA
*
Author to whom correspondence should be addressed.
Toxics 2020, 8(4), 95; https://doi.org/10.3390/toxics8040095
Received: 15 September 2020 / Revised: 17 October 2020 / Accepted: 22 October 2020 / Published: 29 October 2020
(This article belongs to the Special Issue Current Knowledge of E-cigarettes and Heated Tobacco Products)
Electronic cigarettes (e-cigs) use has been dramatically increased recently, especially among youths. Previous studies from our laboratory showed that chronic exposure to e-cigs, containing 24 mg/mL nicotine, was associated with dysregulation of glutamate transporters and neurotransmitter levels in the brain of a mouse model. In this study, we evaluated the effect of three months’ continuous exposure to e-cig vapor (JUUL pods), containing a high nicotine concentration, on the expression of glutamate receptors and transporters in drug reward brain regions such as the nucleus accumbens (NAc) core (NAc-core), NAc shell (NAc-shell) and hippocampus (HIP) in female C57BL/6 mice. Three months’ exposure to mint- or mango-flavored JUUL (containing 5% nicotine, 59 mg/mL) induced upregulation of metabotropic glutamate receptor 1 (mGluR1) and postsynaptic density protein 95 (phosphorylated and total PSD95) expression, and downregulation of mGluR5 and glutamate transporter 1 (GLT-1) in the NAc-shell. In addition, three months’ exposure to JUUL was associated with upregulation of mGluR5 and GLT-1 expression in the HIP. These findings demonstrated that three-month exposure to e-cig vapor containing high nicotine concentrations induced differential effects on the glutamatergic system in the NAc and HIP, suggesting dysregulation of glutamatergic system activity in mesolimbic brain regions. View Full-Text
Keywords: e-cigarettes; nicotine; JUUL; mGluR1; mGluR5; GLT-1; xCT; nucleus accumbens; hippocampus e-cigarettes; nicotine; JUUL; mGluR1; mGluR5; GLT-1; xCT; nucleus accumbens; hippocampus
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MDPI and ACS Style

Alhaddad, H.; Wong, W.; Sari, A.T.; Crotty Alexander, L.E.; Sari, Y. Effects of 3-Month Exposure to E-Cigarette Aerosols on Glutamatergic Receptors and Transporters in Mesolimbic Brain Regions of Female C57BL/6 Mice. Toxics 2020, 8, 95. https://doi.org/10.3390/toxics8040095

AMA Style

Alhaddad H, Wong W, Sari AT, Crotty Alexander LE, Sari Y. Effects of 3-Month Exposure to E-Cigarette Aerosols on Glutamatergic Receptors and Transporters in Mesolimbic Brain Regions of Female C57BL/6 Mice. Toxics. 2020; 8(4):95. https://doi.org/10.3390/toxics8040095

Chicago/Turabian Style

Alhaddad, Hasan, Woonyen Wong, Adam T. Sari, Laura E. Crotty Alexander, and Youssef Sari. 2020. "Effects of 3-Month Exposure to E-Cigarette Aerosols on Glutamatergic Receptors and Transporters in Mesolimbic Brain Regions of Female C57BL/6 Mice" Toxics 8, no. 4: 95. https://doi.org/10.3390/toxics8040095

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