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Open AccessArticle

Toxicological Studies of Czech Beers and Their Constituents

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Department of Genetics, University of Córdoba, 14071 Córdoba, Spain
2
Research and Teaching Brewery, Department of Technological Equipment of Buildings, Faculty of Engineering, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Pargue, Czech Republic
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Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Pargue, Czech Republic
4
Agri-Food Laboratory, CAGPDS, Avda. Menéndez Pidal s/n, 14080, Córdoba, Spain
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Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamýcká 129, 16500 Pargue, Czech Republic
*
Author to whom correspondence should be addressed.
Foods 2019, 8(8), 328; https://doi.org/10.3390/foods8080328
Received: 25 June 2019 / Revised: 3 August 2019 / Accepted: 6 August 2019 / Published: 8 August 2019
(This article belongs to the Special Issue The Health Benefits of Fruits and Vegetables)
Background: Czech beers are unique because they are brewed using specific technology at a particular latitude and for being entirely produced in the area of the Czech Republic. The purpose of this work is the evaluation of toxicological effects of a variety of freeze-dried Czech beers, their raw materials (malts, hops and yeast) and processed-beer (wort, hopped wort and young beer). Methods: In vivo assays to evaluate the safety and protective effects in the Drosophila melanogaster eukaryotic system, and the in vitro evaluations of chemopreventive and DNA damage activity using the HL-60 tumour human cell line were carried out. Results: The safe effects for all the analysed substances and general protective effects against H2O2 were shown both at the individual and genomic level in the Drosophila animal model, with some exceptions. Moreover, all the substances were able to inhibit the tumour cell growth and to induce DNA damage in the HL-60 cells at different levels (proapoptotic, single/double strands breaks and methylation status). Conclusions: The promising effects shown by freeze-dried Czech beers due to their safety, protection against a toxin, chemopreventive potential and the induction of DNA damage in tumour cells, allow the proposition of Czech beer as a beverage with nutraceutic potential. View Full-Text
Keywords: Czech beer; brewing; raw material; Drosophila melanogaster; HL-60 cell line; toxicity; antitoxicity; longevity; cytotoxicity; DNA damage Czech beer; brewing; raw material; Drosophila melanogaster; HL-60 cell line; toxicity; antitoxicity; longevity; cytotoxicity; DNA damage
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Merinas-Amo, T.; Merinas-Amo, R.; García-Zorrilla, V.; Velasco-Ruiz, A.; Chladek, L.; Plachy, V.; del Río-Celestino, M.; Font, R.; Kokoska, L.; Alonso-Moraga, Á. Toxicological Studies of Czech Beers and Their Constituents. Foods 2019, 8, 328.

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