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Open AccessArticle

Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells

1
Rheumatology Unit, Department of Medicine—DIMED, University of Padova, 35128 Padova, Italy
2
Laboratorio de Líquido Sinovial, Instituto Nacional de Rehabilitación “Luis Guillermo Ibarra”, 14380 Mexico City, Mexico
3
Institute of Translational Pharmacology—National Research Council, 00185 Rome, Italy
4
Clinical Nutrition Unit, Department of Medicine—DIMED, University of Padova, 35128 Padova, ltaly
5
Centre for Gout and Metabolic Bone and Joint Diseases, Rheumatology, SS Giovanni and Paolo Hospital, 30122 Venice, Italy
*
Author to whom correspondence should be addressed.
These authors equally contributed to this article.
Foods 2019, 8(11), 560; https://doi.org/10.3390/foods8110560
Received: 21 October 2019 / Revised: 2 November 2019 / Accepted: 5 November 2019 / Published: 7 November 2019
Resveratol (RES) and its natural precursor polydatin (PD) are polyphenols that may display a broad variety of beneficial effects including anti-inflammatory properties. This study aimed to investigate the role of RES and PD in the inflammatory process induced by monosodium urate (MSU) and calcium pyrophosphate (CPP) crystals in vitro. A monocytic cell line (THP-1) was primed for 3 hours with phorbol myristate acetate (100 ng/mL) and stimulated with synthetic MSU (0.05 mg/mL) and CPP (0.025 mg/mL) crystals. RES and PD were added to cultures concurrently with the crystals, or as 2-hour pretreatment. The effect of the two polyphenols was evaluated on intracellular and extracellular IL-1β levels, NACHT-LRRPYD-containing protein-3 (NLRP3) inflammasome expression, reactive oxygen species (ROS) and nitric oxide (NO) production, and the assessment of crystal phagocytosis. RES and PD strongly inhibited IL-1β induced by crystals after cell pretreatment. Cell pretreatment was effective also in reducing IL-1 mRNA expression while no effect was observed on NLRP3 gene expression. RES and PD had no effect on crystal phagocytosis when used as pretreatment. Both polyphenols were significantly effective in inhibiting ROS and NO production. Our results demonstrated that RES and PD are effective in inhibiting crystal-induced inflammation. Data obtained after cell pretreatment allow us to hypothesize that these polyphenols act on specific signaling pathways, preventing inflammation. View Full-Text
Keywords: polydatin; resveratrol; urate crystals; pyrophosphate crystals; crystal-induced inflammation polydatin; resveratrol; urate crystals; pyrophosphate crystals; crystal-induced inflammation
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Oliviero, F.; Zamudio-Cuevas, Y.; Belluzzi, E.; Andretto, L.; Scanu, A.; Favero, M.; Ramonda, R.; Ravagnan, G.; López-Reyes, A.; Spinella, P.; Punzi, L. Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells. Foods 2019, 8, 560.

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