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Article

Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of Uraria crinita (L.) Desv. ex DC

by
Ping-Chen Tu
1,
Chih-Ju Chan
2,
Yi-Chen Liu
3,
Yueh-Hsiung Kuo
2,4,5,
Ming-Kuem Lin
2,* and
Meng-Shiou Lee
2,*
1
Program for Cancer Biology and Drug Discovery, China Medical University and Academia Sinica, Taichung 404, Taiwan
2
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan
3
Institute of Biomedical Science and Rong Hsing Research Center for Translational Medicine, National Chung-Hsing University, Taichung 402, Taiwan
4
Department of Biotechnology, Asia University, Taichung 413, Taiwan
5
Chinese Medicine Research Center, China Medical University, Taichung 404, Taiwan
*
Authors to whom correspondence should be addressed.
Foods 2019, 8(11), 543; https://doi.org/10.3390/foods8110543
Submission received: 7 August 2019 / Revised: 3 October 2019 / Accepted: 10 October 2019 / Published: 3 November 2019
(This article belongs to the Special Issue Development of Food Chemistry, Natural Products, and Nutrition Research)
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Abstract

Uraria crinita is used as a functional food ingredient. Little is known about the association between its immunomodulatory activity and its metabolites. We applied a precise strategy for screening metabolites using immunomodulatory fractions from a U. crinata root methanolic extract (UCME) in combination with bioactivity-guided fractionation and NMR-based identification. The fractions from UCME were evaluated in terms of their inhibitory activity against the production of pro-inflammatory cytokines (IL-6 and TNF-α) by lipopolysaccharide (LPS)-stimulated mouse bone marrow-derived dendritic cells (BMDC). The role of the isoflavone genistein was indicated by the 1H NMR profiling of immunomodulatory subfractions (D-4 and D-5) and supported by the result that genistein-knockout subfractions (D-4 w/o and D-5 w/o) had a lower inhibitory activity compared to genistein-containing subfractions. This study suggests that genistein contributes to the immunomodulatory activity of UCME and will help in the standardization of functional food.
Keywords: Uraria crinita; isoflavone; genistein; NMR-based identification; dendritic cells Uraria crinita; isoflavone; genistein; NMR-based identification; dendritic cells
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MDPI and ACS Style

Tu, P.-C.; Chan, C.-J.; Liu, Y.-C.; Kuo, Y.-H.; Lin, M.-K.; Lee, M.-S. Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of Uraria crinita (L.) Desv. ex DC. Foods 2019, 8, 543. https://doi.org/10.3390/foods8110543

AMA Style

Tu P-C, Chan C-J, Liu Y-C, Kuo Y-H, Lin M-K, Lee M-S. Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of Uraria crinita (L.) Desv. ex DC. Foods. 2019; 8(11):543. https://doi.org/10.3390/foods8110543

Chicago/Turabian Style

Tu, Ping-Chen, Chih-Ju Chan, Yi-Chen Liu, Yueh-Hsiung Kuo, Ming-Kuem Lin, and Meng-Shiou Lee. 2019. "Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of Uraria crinita (L.) Desv. ex DC" Foods 8, no. 11: 543. https://doi.org/10.3390/foods8110543

APA Style

Tu, P.-C., Chan, C.-J., Liu, Y.-C., Kuo, Y.-H., Lin, M.-K., & Lee, M.-S. (2019). Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of Uraria crinita (L.) Desv. ex DC. Foods, 8(11), 543. https://doi.org/10.3390/foods8110543

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