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Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of Uraria crinita (L.) Desv. ex DC

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Program for Cancer Biology and Drug Discovery, China Medical University and Academia Sinica, Taichung 404, Taiwan
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Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan
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Institute of Biomedical Science and Rong Hsing Research Center for Translational Medicine, National Chung-Hsing University, Taichung 402, Taiwan
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Department of Biotechnology, Asia University, Taichung 413, Taiwan
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Chinese Medicine Research Center, China Medical University, Taichung 404, Taiwan
*
Authors to whom correspondence should be addressed.
Foods 2019, 8(11), 543; https://doi.org/10.3390/foods8110543
Received: 7 August 2019 / Revised: 3 October 2019 / Accepted: 10 October 2019 / Published: 3 November 2019
Uraria crinita is used as a functional food ingredient. Little is known about the association between its immunomodulatory activity and its metabolites. We applied a precise strategy for screening metabolites using immunomodulatory fractions from a U. crinata root methanolic extract (UCME) in combination with bioactivity-guided fractionation and NMR-based identification. The fractions from UCME were evaluated in terms of their inhibitory activity against the production of pro-inflammatory cytokines (IL-6 and TNF-α) by lipopolysaccharide (LPS)-stimulated mouse bone marrow-derived dendritic cells (BMDC). The role of the isoflavone genistein was indicated by the 1H NMR profiling of immunomodulatory subfractions (D-4 and D-5) and supported by the result that genistein-knockout subfractions (D-4 w/o and D-5 w/o) had a lower inhibitory activity compared to genistein-containing subfractions. This study suggests that genistein contributes to the immunomodulatory activity of UCME and will help in the standardization of functional food. View Full-Text
Keywords: Uraria crinita; isoflavone; genistein; NMR-based identification; dendritic cells Uraria crinita; isoflavone; genistein; NMR-based identification; dendritic cells
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MDPI and ACS Style

Tu, P.-C.; Chan, C.-J.; Liu, Y.-C.; Kuo, Y.-H.; Lin, M.-K.; Lee, M.-S. Bioactivity-Guided Fractionation and NMR-Based Identification of the Immunomodulatory Isoflavone from the Roots of Uraria crinita (L.) Desv. ex DC. Foods 2019, 8, 543.

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