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Open AccessArticle

Secondary Metabolites Profiling, Biological Activities and Computational Studies of Abutilon figarianum Webb (Malvaceae)

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School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway Selangor Darul Ehsan 47500, Malaysia
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Institute of Pharmaceutical Sciences (IPS), University of Veterinary and Animal Sciences (UVAS), Lahore 54000, Pakistan
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College of Pharmacy, Al Ain University, Al Ain 64141, UAE
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Department of Pharmacology, CMH Institute of Medical Sciences, National University of Medical Sciences (NUMS), Bahawalpur 63100, Pakistan
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Department of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan
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Department of Biology, Faculty of Science, Selcuk University, Campus/Konya 42130, Turkey
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Department of Pharmacy, University ‘G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, Italy
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Chemistry Department, College of Education, Salahaddin University, Erbil 44002, Iraq
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Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam
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Department of Health Sciences, Faculty of Science, University of Mauritius, Réduit 80837, Mauritius
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Department of Bioresources and Food Science, Konkuk University, Seoul 05029, Korea
*
Authors to whom correspondence should be addressed.
Processes 2020, 8(3), 336; https://doi.org/10.3390/pr8030336
Received: 6 February 2020 / Revised: 5 March 2020 / Accepted: 6 March 2020 / Published: 13 March 2020
(This article belongs to the Special Issue Production and Biomedical Applications of Bioactive Compounds)
This research endeavors to inspect the chemical and biological profiling of methanol and dichloromethane (DCM) extracts prepared from Abutilon figarianum Webb. Total bioactive constituents and secondary metabolites were assessed via ultra-high performance liquid chromatography (UHPLC-MS). Biological effects were evaluated via antioxidant and enzymes inhibitory assays. The methanol extract was able to give the highest phenolic (51.92 mg GAE/g extract) and flavonoid (72.59 mg QE/g extract) contents and was found to contain 11 bioactive metabolites, including flavonoid, alkaloid, phenolic and fatty acid derivatives, as accessed by UHPLC-MS analysis. Similarly, the phytochemical profiling of the DCM extract tentatively identified the 12 different secondary metabolites, most of these were fatty acid derivatives. The methanol extract was most active in the radical scavenging, reducing power and total antioxidant power assays, while dichloromethane extract showed the highest metal chelating activity. For enzyme inhibition, the DCM extract showed the highest activity against cholinesterases, glucosidase and amylase, whereas methanol extract was most active against tyrosinase. Docking studies have supported the observed biological activity, where isobergapten showed higher activity against tyrosinase (−7.63 kcal/mol) with inhibition constant (2.55 µM), as opposed to other enzymes. The observed antioxidant and inhibitory potentials of A. figarianum against the studied enzymes tend to endorse this plant as a prospective source of bioactive phytochemicals. View Full-Text
Keywords: phytochemical; docking; antioxidant; enzyme inhibition; bioactive molecules phytochemical; docking; antioxidant; enzyme inhibition; bioactive molecules
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Saleem, H.; Sarfraz, M.; Ahsan, H.M.; Khurshid, U.; Kazmi, S.A.J.; Zengin, G.; Locatelli, M.; Ahmad, I.; Abdallah, H.H.; Mahomoodally, M.F.; Rengasamy, K.R.; Ahemad, N. Secondary Metabolites Profiling, Biological Activities and Computational Studies of Abutilon figarianum Webb (Malvaceae). Processes 2020, 8, 336.

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