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Open AccessFeature PaperReview

Do Bioreactor Designs with More Efficient Oxygen Supply to Ovarian Cortical Tissue Fragments Enhance Follicle Viability and Growth In Vitro?

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Department of Mechanical, Energy and Management Engineering, University of Calabria, 87030 Rende (CS), Italy
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Department of Medical and Surgical Sciences, Magna Graecia University, 88100 Catanzaro, Italy
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Department of Biology, University of Naples Federico II, 80100 Naples, Italy
*
Author to whom correspondence should be addressed.
Processes 2019, 7(7), 450; https://doi.org/10.3390/pr7070450
Received: 17 June 2019 / Revised: 9 July 2019 / Accepted: 10 July 2019 / Published: 15 July 2019
(This article belongs to the Special Issue Feature Review Papers)
Background: Autotransplantation of cryopreserved ovarian tissue is currently the main option to preserve fertility for cancer patients. To avoid cancer cell reintroduction at transplantation, a multi-step culture system has been proposed to obtain fully competent oocytes for in vitro fertilization. Current in vitro systems are limited by the low number and health of secondary follicles produced during the first step culture of ovarian tissue fragments. To overcome such limitations, bioreactor designs have been proposed to enhance oxygen supply to the tissue, with inconsistent results. This retrospective study investigates, on theoretical grounds, whether the lack of a rational design of the proposed bioreactors prevented the full exploitation of follicle growth potential. Methods: Models describing oxygen transport in bioreactors and tissue were developed and used to predict oxygen availability inside ovarian tissue in the pertinent literature. Results: The proposed theoretical analysis suggests that a successful outcome is associated with enhanced oxygen availability in the cultured tissue in the considered bioreactor designs. This suggests that a rational approach to bioreactor design for ovarian tissue culture in vitro may help exploit tissue potential to support follicle growth. View Full-Text
Keywords: bioreactor; design; in vitro culture; ovarian tissue; oxygen; transport bioreactor; design; in vitro culture; ovarian tissue; oxygen; transport
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Catapano, G.; Fragomeni, G.; Falvo D’Urso Labate, G.; De Napoli, L.; Barbato, V.; Di Nardo, M.; Costanzo, V.; Capriglione, T.; Gualtieri, R.; Talevi, R. Do Bioreactor Designs with More Efficient Oxygen Supply to Ovarian Cortical Tissue Fragments Enhance Follicle Viability and Growth In Vitro? Processes 2019, 7, 450.

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