Next Article in Journal / Special Issue
Carbon Nanostructures as a Multi-Functional Platform for Sensing Applications
Previous Article in Journal
Propylene Glycol Stabilizes the Linear Response of Glutamate Biosensor: Potential Implications for In-Vivo Neurochemical Monitoring
Previous Article in Special Issue
Nanostructured Nickel on Porous Carbon-Silica Matrix as an Efficient Electrocatalytic Material for a Non-Enzymatic Glucose Sensor
Article Menu

Export Article

Open AccessReview
Chemosensors 2018, 6(4), 59; https://doi.org/10.3390/chemosensors6040059

Functionalization of Bulk SiO2 Surface with Biomolecules for Sensing Applications: Structural and Functional Characterizations

1
CNR-IMM Headquarters, Strada VIII Z.I. 5, 95121 Catania, Italy
2
Department of Physics and Astronomy, University of Catania, 95100 Catania, Italy
3
STMicroelectronics, Stradale Primosole 50, 95121 Catania, Italy
4
Department of Biomedical and Biotechnological Sciences, Via S. Sofia 87, 95100 Catania, Italy
*
Author to whom correspondence should be addressed.
Received: 31 October 2018 / Revised: 27 November 2018 / Accepted: 27 November 2018 / Published: 30 November 2018
(This article belongs to the Special Issue Nanotechnology Efforts for Chemical Sensors)
  |  
PDF [7646 KB, uploaded 14 December 2018]
  |  

Abstract

Biomolecule immobilization on bulk silicon dioxide (SiO2) is an important aspect in the field of Si-based interfaces for biosensing. The approach used for surface preparation should guarantee not only the stable anchoring of biomolecules but also their structural integrity and biological functioning. In this paper, we review our findings on the SiO2 functionalization process to immobilize a variety of biomolecules, including glucose oxidase, horseradish peroxide, metallothionein, and DNA molecules. Morphological and chemical characterization of SiO2 surfaces after biomolecule immobilization using techniques already employed in the microelectronic industry are presented and discussed. Optical and spectrophotometric analysis revealed the preservation of biomolecules’ activity once they are anchored on the biointerface. View Full-Text
Keywords: biointerface; silicon; ssDNA; glucose oxidase; horseradish peroxide; metallothionein; TEM; XPS biointerface; silicon; ssDNA; glucose oxidase; horseradish peroxide; metallothionein; TEM; XPS
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Sciuto, E.L.; Bongiorno, C.; Scandurra, A.; Petralia, S.; Cosentino, T.; Conoci, S.; Sinatra, F.; Libertino, S. Functionalization of Bulk SiO2 Surface with Biomolecules for Sensing Applications: Structural and Functional Characterizations. Chemosensors 2018, 6, 59.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Chemosensors EISSN 2227-9040 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top