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Article

Polyphenol Characterization and Skin-Preserving Properties of Hydroalcoholic Flower Extract from Himantoglossum robertianum (Orchidaceae)

1
Department of Earth, Environment and Life Sciences, University of Genova, 16132 Genova, Italy
2
Department of Pharmacy, University of Genova, 16132 Genova, Italy
3
Institute of Biophysics, National Research Council (CNR), 16149 Rome, Italy
4
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy
*
Author to whom correspondence should be addressed.
Plants 2019, 8(11), 502; https://doi.org/10.3390/plants8110502
Received: 20 October 2019 / Revised: 10 November 2019 / Accepted: 12 November 2019 / Published: 14 November 2019
(This article belongs to the Special Issue Bioactive Compounds in Plants)
Himantoglossum robertianum (Loisel.) P. Delforge is a Mediterranean orchid whose propagation in vitro has been achieved, making it eligible as a source of bioactive substances. Flowers were analyzed by light and SEM microscopy and used to obtain a polyphenol-rich, hydroalcoholic flower extract (HFE). HFE was characterized for total phenols, flavonoids and proanthocyanidins, and for polyphenol profile by RP-LC-DAD. Antioxidant assays, in vitro collagenase and elastase inhibition, and MTT and cell motility assays on HaCaT keratinocytes were done. Microscopy showed epidermal cells containing anthocyanins in the flower labellum. Flavonoids (flavones and flavan-3-ols) represented the most abundant compounds (42.91%), followed by scopoletin (33.79%), and phenolic acids (23.3%). Antioxidant assays showed strong activities, rating ORAC > FRAP > TEAC > β-carotene bleaching > DPPH > iron-chelation. Biological assays showed elastase and collagenase inhibition (up to 42% and 78%, respectively), improvement of HaCaT cell viability after treatment with 500 μM H2O2 (from 30% to 84% of control), and stimulation of cell migration rate up to 210% of control. In summary, HFE counteracted different free radicals, while protective properties were shown by cell-free and cell-based bioassays, suggesting the possible use of H. robertianum flowers for skin-preserving, repair, and anti-aging applications. View Full-Text
Keywords: antioxidants; collagenase; elastase; flavonoids; keratinocytes; skin aging; Himantoglossum robertianum antioxidants; collagenase; elastase; flavonoids; keratinocytes; skin aging; Himantoglossum robertianum
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MDPI and ACS Style

Bazzicalupo, M.; Burlando, B.; Denaro, M.; Barreca, D.; Trombetta, D.; Smeriglio, A.; Cornara, L. Polyphenol Characterization and Skin-Preserving Properties of Hydroalcoholic Flower Extract from Himantoglossum robertianum (Orchidaceae). Plants 2019, 8, 502. https://doi.org/10.3390/plants8110502

AMA Style

Bazzicalupo M, Burlando B, Denaro M, Barreca D, Trombetta D, Smeriglio A, Cornara L. Polyphenol Characterization and Skin-Preserving Properties of Hydroalcoholic Flower Extract from Himantoglossum robertianum (Orchidaceae). Plants. 2019; 8(11):502. https://doi.org/10.3390/plants8110502

Chicago/Turabian Style

Bazzicalupo, Miriam; Burlando, Bruno; Denaro, Marcella; Barreca, Davide; Trombetta, Domenico; Smeriglio, Antonella; Cornara, Laura. 2019. "Polyphenol Characterization and Skin-Preserving Properties of Hydroalcoholic Flower Extract from Himantoglossum robertianum (Orchidaceae)" Plants 8, no. 11: 502. https://doi.org/10.3390/plants8110502

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