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Open AccessCommunication

Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon

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University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech Republic
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University of Vienna, Department of Theoretical Biology, Althanstraße 14, 1090 Vienna, Austria
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(8), 333; https://doi.org/10.3390/biom9080333
Received: 1 July 2019 / Revised: 30 July 2019 / Accepted: 1 August 2019 / Published: 1 August 2019
(This article belongs to the Special Issue Relevant Biomolecules for Germ Cells and Fertilization)
Nanoparticles are finding increasing applications in diagnostics, imaging and therapeutics in medicine. Iron oxide nanoparticles (IONs) have received significant interest of scientific community due to their distinctive properties. For the first time, we have delivered IONs into germ cells in any species. Our results showed that sturgeon primordial germ cells (PGCs) delivered with IONs could be detected until seven days post fertilization (dpf) under fluorescent microscope and at 22 dpf by micro-CT. Delivery of IONs into cells could be helpful for studying germ cell biology and the improvement of germ cell-based bio-technologies as isolation of PGCs using magnetic activated cell sorting or application of hyperthermia for a host sterilization purpose. Intriguingly, in our study, we did not find any toxic effects of IONs on the survival and hatching rates of sturgeon embryos when compared with embryos injected with FITC-dextran only. View Full-Text
Keywords: acipenser; caviar; hyperthermia; iron oxide nanoparticles; micro-CT; sterilization acipenser; caviar; hyperthermia; iron oxide nanoparticles; micro-CT; sterilization
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Baloch, A.R.; Fučíková, M.; Rodina, M.; Metscher, B.; Tichopád, T.; Shah, M.A.; Franěk, R.; Pšenička, M. Delivery of Iron Oxide Nanoparticles into Primordial Germ Cells in Sturgeon. Biomolecules 2019, 9, 333.

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