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Open AccessArticle

Embryonic Exposure to Bisphenol A Impairs Primordial Germ Cell Migration without Jeopardizing Male Breeding Capacity

1
Department of Molecular Biology, Faculty of Biology and Environmental Sciences, Campus de Vegazana, Universidad de León, 24071 León, Spain
2
Fish Physiology and Genomics Department, Campus de Beaulieu, INRA, 35000 Rennes, France
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(8), 307; https://doi.org/10.3390/biom9080307
Received: 26 June 2019 / Revised: 21 July 2019 / Accepted: 22 July 2019 / Published: 25 July 2019
(This article belongs to the Special Issue Relevant Biomolecules for Germ Cells and Fertilization)
A large amount of chemicals are released to the environment each year. Among them, bisphenol A (BPA) is of utmost concern since it interferes with the reproductive system of wild organisms due to its capacity to bind to hormone receptors. Additionally, BPA epigenotoxic activity is known to affect basic processes during embryonic life. However, its effects on primordial germ cells (PGCs) proliferation and migration, both mechanisms being crucial for gametogenesis, remain unknown. To investigate the effects of BPA on PGCs migration and eventual testicle development, zebrafish embryos were exposed to 100, 2000 and 4000 µg/L BPA during the first 24 h of development. Vasa immunostaining of PGCs revealed that exposure to 2000 and 4000 µg/L BPA impaired their migration to the genital ridge. Two pivotal genes of PGCs migration (cxcr4b and sdf1a) were highly dysregulated in embryos exposed to these doses, whereas DNA methylation and epigenetic marks in PGCs and their surrounding somatic cells were not altered. Once embryos reached adulthood, the morphometric study of their gonads revealed that, despite the reduced number of PGCs which colonized the genital ridges, normal testicles were developed. Although H3K9ac decreased in the sperm from treated fishes, it did not affect the progeny development. View Full-Text
Keywords: bisphenol A; primordial germ cells; epigenetics; reproduction; zebrafish bisphenol A; primordial germ cells; epigenetics; reproduction; zebrafish
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MDPI and ACS Style

Lombó, M.; Getino-Álvarez, L.; Depincé, A.; Labbé, C.; Herráez, M.P. Embryonic Exposure to Bisphenol A Impairs Primordial Germ Cell Migration without Jeopardizing Male Breeding Capacity. Biomolecules 2019, 9, 307.

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