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Open AccessArticle

Long Exposure to a Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality and Progeny Survival in the Zebrafish Model

1
IEO, Spanish Institute of Oceanography, Planta de Cultivos El Bocal, Barrio Corbanera, 39012 Monte, Santander, Spain
2
MODCELL Group, Department of Molecular Biology, Universidad de León, 24071 León, Spain
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(8), 338; https://doi.org/10.3390/biom9080338
Received: 1 July 2019 / Revised: 30 July 2019 / Accepted: 1 August 2019 / Published: 3 August 2019
(This article belongs to the Special Issue Relevant Biomolecules for Germ Cells and Fertilization)
The aim of the present experiment is to study the effects of oral ingestion of a mixture of two probiotic bacteria on sperm quality and progenies. Three homogeneous groups of juvenile zebrafish were created. Once having reached adulthood (3 months postfertilization; mpf), each group received different feeding regimens: a standard diet (control), a maltodextrin-supplemented diet (vehicle control), or a probiotic-supplemented diet (a mixture (1:1) of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347). The feeding regime lasted 4.5 months. Growth parameters (weight and length) were determined at 3, 5, and 7.5 mpf. Sperm motility was evaluated using computer-assisted sperm analysis at 5 and 7.5 mpf. Progeny survival, hatching rate, and malformation rate were also evaluated. Results showed that probiotic-supplemented diet improved growth parameters compared with the standard diet. The highest percentage of motile spermatozoa was reported in the probiotic-fed group. Concomitantly, the percentage of fast sperm subpopulation was significantly lower in samples derived from control males. Furthermore, there was a significant difference in progeny survival between the probiotic-fed group and the control group at three developmental times (24 hours postfertilization (hpf), 5 days postfertilization (dpf) and 7 dpf). In conclusion, in zebrafish, prolonged ingestion of a mixture of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347 has positive effects on growth, sperm quality, and progeny survival. View Full-Text
Keywords: spermatozoa; probiotics; embryo; reproduction; nutrition spermatozoa; probiotics; embryo; reproduction; nutrition
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Valcarce, D.G.; Riesco, M.F.; Martínez-Vázquez, J.M.; Robles, V. Long Exposure to a Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality and Progeny Survival in the Zebrafish Model. Biomolecules 2019, 9, 338.

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