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Open AccessArticle

Endo-β-1,3-glucanase (GH16 Family) from Trichoderma harzianum Participates in Cell Wall Biogenesis but Is Not Essential for Antagonism Against Plant Pathogens

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Department of Biochemistry and Cellular Biology, Biological Sciences Institute, Campus Samambaia, Federal University of Goiás (UFG), Goiânia, Goiás 74090-900, Brazil
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Department of Physiological Sciences, Health Sciences Centre, Federal University of Espirito Santo, Vitoria ES 29047-105, Brazil
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Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo (USP), Ribeirão Preto 14049-900, Brazil
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Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, Ribeirão Preto CEP 14040-903, Brazil
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Department of Cell Biology, University of Brasília, Campus Darcy Ribeiro, Biological Sciences Institute, Brasília CEP 70.910-900, Brazil
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Campus of Exact Sciences and Technologies, Campus Henrique Santillo, State University of Goiás (UEG), BR 153, Km 98, Anápolis 75000-000, Brazil
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Authors to whom correspondence should be addressed.
Biomolecules 2019, 9(12), 781; https://doi.org/10.3390/biom9120781
Received: 17 October 2019 / Revised: 20 November 2019 / Accepted: 20 November 2019 / Published: 26 November 2019
(This article belongs to the Special Issue Biology, Biotechnology and Bioprospecting of Microbial Biomolecules)
Trichoderma species are known for their ability to produce lytic enzymes, such as exoglucanases, endoglucanases, chitinases, and proteases, which play important roles in cell wall degradation of phytopathogens. β-glucanases play crucial roles in the morphogenetic-morphological process during the development and differentiation processes in Trichoderma species, which have β-glucans as the primary components of their cell walls. Despite the importance of glucanases in the mycoparasitism of Trichoderma spp., only a few functional analysis studies have been conducted on glucanases. In the present study, we used a functional genomics approach to investigate the functional role of the gluc31 gene, which encodes an endo-β-1,3-glucanase belonging to the GH16 family in Trichoderma harzianum ALL42. We demonstrated that the absence of the gluc31 gene did not affect the in vivo mycoparasitism ability of mutant T. harzianum ALL42; however, gluc31 evidently influenced cell wall organization. Polymer measurements and fluorescence microscopy analyses indicated that the lack of the gluc31 gene induced a compensatory response by increasing the production of chitin and glucan polymers on the cell walls of the mutant hyphae. The mutant strain became more resistant to the fungicide benomyl compared to the parental strain. Furthermore, qRT-PCR analysis showed that the absence of gluc31 in T. harzianum resulted in the differential expression of other glycosyl hydrolases belonging to the GH16 family, because of functional redundancy among the glucanases. View Full-Text
Keywords: Trichoderma harzianum; endo-β-1,3-glucanase; cell wall; glycosyl hydrolase family 16; gluc31 gene Trichoderma harzianum; endo-β-1,3-glucanase; cell wall; glycosyl hydrolase family 16; gluc31 gene
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Suriani Ribeiro, M.; Graciano de Paula, R.; Raquel Voltan, A.; de Castro, R.G.; Carraro, C.B.; José de Assis, L.; Stecca Steindorff, A.; Goldman, G.H.; Silva, R.N.; Ulhoa, C.J.; Neves Monteiro, V. Endo-β-1,3-glucanase (GH16 Family) from Trichoderma harzianum Participates in Cell Wall Biogenesis but Is Not Essential for Antagonism Against Plant Pathogens. Biomolecules 2019, 9, 781.

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