Cerebral Ischemic Preconditioning Aggravates Death of Oligodendrocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Middle Cerebral Artery Occlusion
2.3. Measurements of Infarct Volume
2.4. Primary Cell Culture
2.5. Lentivirus Transfection, Oxygen-Glucose Deprivation (OGD), and Pharmacological Treatment
2.6. Cell Counts and Viability Assay
2.7. Western Blots
2.8. Immunofluorescent Staining, TUNEL, and Microscopy
2.9. Toluidine Blue Staining and Transmission Electron Microscopy (TEM)
2.10. Statistical Analysis
3. Results
3.1. Preconditioning Does Not Prevent Striatal Infarct after Ischemic Stroke
3.2. BNIP3 Knockout Mice Have Less Cerebral Damage Compared with Wild-Type Mice
3.3. BNIP3 Knockout Rescues Myelination Defects after Preconditioning
3.4. BNIP3 Is Associated with OPC Vulnerability in Preconditioning
3.5. Preconditioning Triggered Oligodendrocytic MCT1 Loss, and the Toxicity to OPC Is Attenuated by Knocking down BNIP3
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Guan, T.; Guo, Y.; Li, C.; Zhou, T.; Yu, Q.; Yang, C.; Zhang, G.; Kong, J. Cerebral Ischemic Preconditioning Aggravates Death of Oligodendrocytes. Biomolecules 2022, 12, 1872. https://doi.org/10.3390/biom12121872
Guan T, Guo Y, Li C, Zhou T, Yu Q, Yang C, Zhang G, Kong J. Cerebral Ischemic Preconditioning Aggravates Death of Oligodendrocytes. Biomolecules. 2022; 12(12):1872. https://doi.org/10.3390/biom12121872
Chicago/Turabian StyleGuan, Teng, Ying Guo, Chengren Li, Ting Zhou, Qiang Yu, Chaoxian Yang, Guohui Zhang, and Jiming Kong. 2022. "Cerebral Ischemic Preconditioning Aggravates Death of Oligodendrocytes" Biomolecules 12, no. 12: 1872. https://doi.org/10.3390/biom12121872