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Article

Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju

1
Department of Functional Anatomy and Cytobiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, Poland
2
Department of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, Poland
3
Department of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, Poland
4
Department of Experimental Hematooncology, Medical University of Lublin, 20-031 Lublin, Poland
5
Department of General Ophthalmology, Medical University of Lublin, 20-031 Lublin, Poland
*
Authors to whom correspondence should be addressed.
Academic Editor: Natália Martins
Biomolecules 2021, 11(8), 1089; https://doi.org/10.3390/biom11081089
Received: 23 June 2021 / Revised: 19 July 2021 / Accepted: 20 July 2021 / Published: 23 July 2021
(This article belongs to the Special Issue Fungal Metabolism - Enzymes and Bioactive Compounds)
The present study aimed to demonstrate Lentinus (formerly Pleurotus) sajor-caju (PSC) as a good source of pro-health substances. It has also shown that supplementation of its culture medium with cow milk may further improve its beneficial properties. Intracellular fractions from fungi grown on a medium supplemented with cow milk were analyzed using various biochemical methods for determination of the nutrient composition. Furthermore, anti-cancer properties of selected extracts were investigated on colorectal cancer cell lines (HT-29, LS 180, and SW948) in vitro. Biochemical analysis showed enrichment in health-enhancing compounds, such as proteins or polysaccharides (about 3.5- and 4.5-fold increase in concentration of proteins and carbohydratesin extracts of mycelia cultured on whole milk (PSC2-I), respectively), with a decrease in the level of free radicals (10-fold decrease in extract grown on milk and medium mixture (1:1) (PSC3-II)), which was related to increased catalase and superoxide dismutase activity (7.5-fold increase in catalase activity and 5-fold in SOD activity in PSC3-II compared to the control). Moreover, the viability of the cancer cells was diminished (to 60.0 ± 6.8% and 40.0 ± 8.6% of the control, on HT-29 and SW948 cells, respectively), along with pro-apoptotic (to 18.8 ± 11.8 and 14.7 ± 8.0% towards LS 180 and SW948 cells, respectively) and NO-secreting effects (about 2-fold increase) of the extracts. This study suggests that PSC has multiple nutritional and anti-cancer properties and can be used as a source of healthy biomolecules in modern medicine or functional foods. View Full-Text
Keywords: fungi; arboreal medicinal mushrooms; Pleurotus sajor-caju; Lentinus sajor-caju; bioactive compounds; colorectal cancer; anti-cancer activity; milk supplementation fungi; arboreal medicinal mushrooms; Pleurotus sajor-caju; Lentinus sajor-caju; bioactive compounds; colorectal cancer; anti-cancer activity; milk supplementation
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MDPI and ACS Style

Zając, A.; Pięt, M.; Stefaniuk, D.; Chojnacki, M.; Jakubowicz-Gil, J.; Paduch, R.; Matuszewska, A.; Jaszek, M. Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju. Biomolecules 2021, 11, 1089. https://doi.org/10.3390/biom11081089

AMA Style

Zając A, Pięt M, Stefaniuk D, Chojnacki M, Jakubowicz-Gil J, Paduch R, Matuszewska A, Jaszek M. Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju. Biomolecules. 2021; 11(8):1089. https://doi.org/10.3390/biom11081089

Chicago/Turabian Style

Zając, Adrian, Mateusz Pięt, Dawid Stefaniuk, Michał Chojnacki, Joanna Jakubowicz-Gil, Roman Paduch, Anna Matuszewska, and Magdalena Jaszek. 2021. "Pro-Health and Anti-Cancer Activity of Fungal Fractions Isolated from Milk-Supplemented Cultures of Lentinus (Pleurotus) Sajor-caju" Biomolecules 11, no. 8: 1089. https://doi.org/10.3390/biom11081089

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