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Article

Thromboelastometric Analysis of Anticancer Cerrena unicolor Subfractions Reveal Their Potential as Fibrin Glue Drug Carrier Enhancers

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Department of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-033 Lublin, Poland
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Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Bialystok, 15-089 Białystok, Poland
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Department of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-033 Lublin, Poland
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Department of Functional Anatomy and Cytobiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-033 Lublin, Poland
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Department of Paediatric Orthopaedics and Rehabilitation, Faculty of Medicine, Medical University of Lublin, 20-093 Lublin, Poland
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Department of Biopharmacy, Faculty of Pharmacy, Medical University of Bialystok, 15-089 Białystok, Poland
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Chair and Department of Biochemistry and Biotechnology, Faculty of Pharmacy, Medical University of Lublin, 20-093 Lublin, Poland
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Department of Biochemistry, Institute of Soil Science and Plant Cultivation—State Research Institute, 24-100 Puławy, Poland
*
Authors to whom correspondence should be addressed.
Academic Editor: Natália Martins
Biomolecules 2021, 11(9), 1263; https://doi.org/10.3390/biom11091263
Received: 30 June 2021 / Revised: 19 August 2021 / Accepted: 20 August 2021 / Published: 24 August 2021
(This article belongs to the Special Issue Fungal Metabolism - Enzymes and Bioactive Compounds)
In this study, the influence of two subfractions (with previously proven anti-cancer properties) isolated from wood rot fungus Cerrena unicolor on the formation of a fibrin clot was investigated in the context of potential use as fibrin glue and sealant enhancers and potential wound healing agents. With the use of ROTEM thromboelastometry, we demonstrated that, in the presence of fibrinogen and thrombin, the S6 fraction accelerated the formation of a fibrin clot, had a positive effect on its elasticity modulus, and enhanced the degree of fibrin cross-linking. The S5 fraction alone showed no influence on the fibrin coagulation process; however, in the presence of fibrin, it exhibited a decrease in anti-proliferative properties against the HT-29 line, while it increased the proliferation of cells in general at a concentration of 100 µg/mL. Both fractions retained their proapoptotic properties to a lesser degree. In combination with the S6 fraction in the ratio of 1:1 and 1:3, the fractions contributed to increased inhibition of the activity of matrix metalloproteinases (MMPs). This may suggest anti-metastatic activity of the combined fractions. In conclusion, the potential of the fractions isolated from the C. unicolor secretome to be used as a means of improving the wound healing process was presented. The potential for delivering agents with cytostatic properties introduced far from the site of action or exerting a pro-proliferative effect at the wound site with the aid of a fibrin sealant was demonstrated. View Full-Text
Keywords: Cerrena unicolor; CCD 841 CoTr; HT-29; fibrin coagulation; fibrin-based sealant; low molecular weight subfractions Cerrena unicolor; CCD 841 CoTr; HT-29; fibrin coagulation; fibrin-based sealant; low molecular weight subfractions
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MDPI and ACS Style

Stefaniuk, D.; Misztal, T.; Pięt, M.; Zając, A.; Kopycińska, M.; Matuszewska, A.; Ruminowicz-Stefaniuk, M.; Matuszewski, Ł.; Marcińczyk, N.; Belcarz, A.; Żuchowski, J.; Skrabalak, I.; Grąz, M.; Ciołek, B.; Paduch, R.; Jaszek, M. Thromboelastometric Analysis of Anticancer Cerrena unicolor Subfractions Reveal Their Potential as Fibrin Glue Drug Carrier Enhancers. Biomolecules 2021, 11, 1263. https://doi.org/10.3390/biom11091263

AMA Style

Stefaniuk D, Misztal T, Pięt M, Zając A, Kopycińska M, Matuszewska A, Ruminowicz-Stefaniuk M, Matuszewski Ł, Marcińczyk N, Belcarz A, Żuchowski J, Skrabalak I, Grąz M, Ciołek B, Paduch R, Jaszek M. Thromboelastometric Analysis of Anticancer Cerrena unicolor Subfractions Reveal Their Potential as Fibrin Glue Drug Carrier Enhancers. Biomolecules. 2021; 11(9):1263. https://doi.org/10.3390/biom11091263

Chicago/Turabian Style

Stefaniuk, Dawid, Tomasz Misztal, Mateusz Pięt, Adrian Zając, Magdalena Kopycińska, Anna Matuszewska, Marta Ruminowicz-Stefaniuk, Łukasz Matuszewski, Natalia Marcińczyk, Anna Belcarz, Jerzy Żuchowski, Ilona Skrabalak, Marcin Grąz, Beata Ciołek, Roman Paduch, and Magdalena Jaszek. 2021. "Thromboelastometric Analysis of Anticancer Cerrena unicolor Subfractions Reveal Their Potential as Fibrin Glue Drug Carrier Enhancers" Biomolecules 11, no. 9: 1263. https://doi.org/10.3390/biom11091263

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