Age and Gender Effects on Genotoxicity in Diesel Exhaust Particles Exposed C57BL/6 Mice

Department of Chronic Disease Convergence Research, Division of Allergy and Respiratory Disease Research, Korea National Institute of Health, Chungju 28159, Korea

GLP Center 1, Korea Conformity Laboratories, Bio Division, Incheon 21999, Korea

Department of Materials Science and Engineering, Yonsei University, Seoul 03722, Korea

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Academic Editors: Je Yu and Joseph Erlichman

Biomolecules 2021, 11(3), 374; https://doi.org/10.3390/biom11030374

Received: 3 January 2021 / Revised: 20 February 2021 / Accepted: 23 February 2021 / Published: 2 March 2021

(This article belongs to the Special Issue Health and Environmental Effect of Advanced Materials and Fine Particles)

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Abstract

There is growing evidence that the accumulation of DNA damage induced by fine particulate matter (PM_2.5) exposure is an underlying mechanism of pulmonary disease onset and progression. However, there is a lack of experimental evidence on whether common factors (age, gender) affect PM_2.5 induced genomic damage. Here, we assessed the DNA damage potency of PM_2.5 using conventional genotoxicity testing in old male and female mice aged 8 and 40 weeks. Mice were intratracheally instilled with diesel exhaust PM_2.5 (DEP, NIST SRM 1650b), twice a week for 4 weeks. Exposure to DEP was not associated with an increase in the frequency of micronucleated polychromatic erythrocytes and did not induce a systemic genotoxic effect in the bone marrow. Meanwhile, the results from the comet assay showed a significant increase in DNA damage in DEP exposed mouse lung specimens. The positive relationship between DEP exposure and DNA damage is stronger in the older than in the younger group. Statistical analysis showed that there was a modifying effect of age on the association between PM_2.5 exposure and DNA damage. Our results suggest that the age factor should be considered to better understand the cellular adverse effects of PM_2.5. View Full-Text

Keywords: fine particulate matter; diesel exhaust particles; genotoxicity; micronucleus assay; comet assay; aged; effect modification

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MDPI and ACS Style

Lee, J.W.; Kim, J.S.; Lee, H.J.; Jang, J.-H.; Kim, J.-H.; Sim, W.J.; Lim, Y.-b.; Jung, J.-W.; Lim, H.J. Age and Gender Effects on Genotoxicity in Diesel Exhaust Particles Exposed C57BL/6 Mice. Biomolecules 2021, 11, 374. https://doi.org/10.3390/biom11030374

AMA Style

Lee JW, Kim JS, Lee HJ, Jang J-H, Kim J-H, Sim WJ, Lim Y-b, Jung J-W, Lim HJ. Age and Gender Effects on Genotoxicity in Diesel Exhaust Particles Exposed C57BL/6 Mice. Biomolecules. 2021; 11(3):374. https://doi.org/10.3390/biom11030374

Chicago/Turabian Style

Lee, Joong W., Jin S. Kim, Hee J. Lee, Ji-Hye Jang, Ja-Hyun Kim, Woo J. Sim, Yong-beom Lim, Ji-Won Jung, and Hyun J. Lim. 2021. "Age and Gender Effects on Genotoxicity in Diesel Exhaust Particles Exposed C57BL/6 Mice" Biomolecules 11, no. 3: 374. https://doi.org/10.3390/biom11030374

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Age and Gender Effects on Genotoxicity in Diesel Exhaust Particles Exposed C57BL/6 Mice

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