Search Results (162,812)

Background: Despite the availability of a killed whole-virus (KV) vaccine, diarrhea caused by equine rotavirus group A (ERVA) remains a significant health concern for foals in the United States. The vaccine is administered to pregnant mares, with foals protected by passive transfer of colostral antibodies. However, KV-induced immunity is only partially protective and maternal antibody levels in foals are often low and wane rapidly. To address these limitations, we developed a mRNA-based ERVA vaccine encoding the highly conserved VP8* protein to evaluate whether it can provide improved immune protection. Methods: Pregnant mares (n=12 per group) were immunized either at months 8 and 10 of gestation with the VP8* mRNA or at months 8, 9, and 10 of gestation with the KV. Serum samples were collected from mares before and after immunization and from their foals at ages 1, 35, and 49 days. Serum samples were tested by indirect ELISA for VP8*-specific relative antibody concentrations and relative concentrations were compared for effects of study group and sample-time using linear mixed-effects regression. To detect functional antibodies against ERVA, a virus neutralization titer assay was performed to compare titers between mares vaccinated with the mRNA vaccine (and their foals) and unvaccinated control mares (and their foals). Results: Mares vaccinated with VP8* mRNA had significantly (P < 0.05) higher antibody concentrations after foaling than mares in the KV group, and foals of VP8* mRNA-vaccinated mares had significantly (P < 0.05) higher concentrations through age 49 days than foals in the KV group. In addition, the VP8* mRNA vaccine elicited higher titers of ERVA-neutralizing antibodies against both G3 and G14 strains. Conclusion: Longer-lasting, higher concentrations of virus-neutralizing antibodies might provide superior duration of immunity to ERVA in foals from mares vaccinated with VP8* mRNA. Full article

Objective: Advanced glycation end-products (AGEs) contribute to oxidative stress and inflammation, leading to various disorders, including skin inflammation. Here, we investigated the anti-inflammatory effects of Aloe vera flower (AVF) extract and its active constituents, vitexin (V) and isovitexin (IV), in a glyoxal-derived AGE (GO-AGE)-induced skin inflammaging model. Methods: We evaluated the effects of AVF, V, and IV in epidermal keratinocytes (HaCaT cells) using enzyme-linked immunosorbent assay, Western blotting, quantitative real-time polymerase chain reaction, and in silico molecular docking. Results: Treatment of HaCaT cells with AVF, V, or IV significantly suppressed the secretion and expression of interleukins (IL-6 and IL-8) at both the mRNA and protein level, and reduced the expression of key inflammatory proteins, including kappa-light-chain-enhancer of activated B cells (NF-κB) and cyclooxygenase-2 (COX-2), and phosphorylation of mitogen-activated protein kinase (MAPK) pathway proteins. Notably, the inhibitory effects of V and IV on COX-2 expression were more comparable to or exceeded those of the positive control (Epigallocatechin gallate), even at a lower concentration. Conversely, the expression of sirtuin 1 (SIRT1) was upregulated by AVF, V, and IV, with IV showing 1.5-fold upregulation. Molecular docking analyses supported these findings, with IV displaying a particularly high binding affinity for COX-2 (−11.0 kcal/mol). Conclusions: These findings highlight the potential of AVF, V, and IV as novel therapeutic agents for managing skin inflammaging by modulating inflammatory pathways. Full article

Gabal (G.) Suwayqat El-Arsha contains two distinct phases of granitoids: I-type granodiorite and A-type monzogranite. Both of them experienced intense fractional crystallization that affected plagioclase, alkali feldspar, quartz, and, to a lesser degree, ferromagnesian minerals. EnMAP hyperspectral data were used to discriminate between the different granitoid types through spectral analysis, using various techniques, including the Sequential Maximum Angle Convex Cone (SMACC) method. Granodiorite has high SiO₂ (68.21–71.44 wt%), Al₂O₃ (14.29–14.92 wt%), Fe₂O₃ (1.99–3.32 wt%), and CaO (2.34–3.87 wt%), whereas monzogranite has even higher SiO₂ (73.58–75.87 wt%) and K₂O (4.28–4.88 wt%). Both granodiorite and monzogranite exhibit calc-alkaline, peraluminous to metaluminous, and medium- to high-K characteristics, with attendant enrichment of light REE and LILE and depletion of heavy REE and HFSE. A negative Eu anomaly may indicate early plagioclase fractionation, especially in the monzogranite. The I-type granodiorite is likely derived from a high-K, mafic protolith that partially melted during lithospheric delamination, leading to severe fractional crystallization in the upper crust in a post-collisional environment. In contrast, the monzogranite exhibits A-type characteristics and was likely emplaced in an anorogenic setting. Both granites were affected by several episodes of hydrothermal alteration, resulting in silicification, kaolinitization, sericitization, and chloritization. The intrusions studied here exhibit key similarities with those in the Wadi El-Hima area, including tectonic setting, petrogenetic type, Neoproterozoic age (Stage I collisional: ca. 650–620 Ma; Stage II post-collisional: ca. 630–590 Ma), and mineralogical assemblages (notably two-mica granites). These correlations suggest that both suites form part of a regionally extensive batholith composed of I- and A-type granites, stretching from north of the Marsa Alam Road (Umm Salatit–Homrit Waggat) southward to at least Wadi El-Hima. Full article

Background: Children with disabilities and special educational needs show heterogeneous developmental profiles that may be associated with executive functioning and family factors. This study examined functional developmental profiles in primary education and their associations with executive functions, behavioural indicators, and parental mental health. Methods: Participants were 106 children aged 6–12 years attending mainstream schools, including a subgroup with special educational needs. Parents completed the Developmental Profile-3 (DP-3) and a family mental-health record, while teachers completed the Screening of Emotional and Behavioural Problems in Children (SPECI) and Behaviour Rating Inventory of Executive Function-2 (BRIEF-2) based on structured ratings derived from daily interaction with the child. Correlational and regression analyses were conducted. Results: Executive functions—particularly working memory and planning/organisation—were consistently associated with global, adaptive, and social development. Parental overload showed negative associations with several developmental domains. Associations with behavioural indicators were modest. Conclusions: Developmental functioning in children with and without special educational needs is associated with executive functions and family factors as perceived by parents and teachers. Findings should be interpreted as relational and inform future assessment and inclusive educational planning. Full article

Background/Objectives: Some Japanese people still adhere to a systematic traditional Japanese diet pattern (the Shokuyo diet) consisting mainly of brown rice, vegetables, legumes, and small amounts of fish. We investigated the impact of this dietary pattern on the intestinal microbiota of its female consumers. Methods: The intestinal microbiota of 19 Japanese females in their 60s and 70s consuming the Shokuyo diet (Shokuyo diet group) and 50 females of the same age consuming a normal Japanese diet (NJ diet group) were compared. The NJ diet group was further subdivided into a healthy NJ diet H subgroup, comprising females (n = 19) without any diseases, and an unhealthy NJ diet UH subgroup (n = 31) consisting of females with certain diseases, and a subgroup analysis was performed. Intestinal microbiota analysis was performed using 16S rRNA gene amplicon sequencing. Results: The β-diversity of the intestinal microbiota significantly differed between the Shokuyo diet and NJ diet groups. Similarly, in the subgroup analysis, β-diversity also significantly differed between the NJ diet UH subgroup and the Shokuyo diet group. However, no significant difference was observed between the NJ diet H and Shokuyo diet groups. These results indicate that the intestinal microbial composition of the Shokuyo diet group resembled that of the healthy participants, and that differences in intestinal microbial composition between the Shokuyo and NJ diet groups were strongly influenced by the presence of participants with diseases in the NJ diet group. That is, differences in β-diversity may have been strongly mediated by the health status of the participants. Conclusions: Consumption of the Shokuyo diet may be associated with a healthy intestinal microbial composition in older Japanese female, suggesting its potential as a viable dietary intervention option. Full article

Background/Objectives: Southeastern Europe and Croatia have served as a genetic crossroads between the Near East and Europe since prehistoric times, shaped by numerous and repeated migrations. By integrating 19 newly generated ancient genomes with 285 previously published ancient genomes from Croatia, we investigated patterns of maternal and paternal landscapes from the Neolithic, Bronze, and Iron Ages through to the Antiquity and medieval periods, as well as the modern Croatian population. Methods: Ancient DNA extraction from human remains and library preparation were conducted in dedicated clean-room facilities, followed by high-throughput sequencing on the Illumina platform. Sequencing data were analyzed with established pipelines to determine mitochondrial and Y-chromosomal haplogroups and the genetic sex of individuals. Results: New ancient data reveal a predominantly European maternal profile, dominated by haplogroups H, U, and HV0, whereas Y-chromosomal lineages are characterized by J subclades and R1a, with limited representation of R1b and the absence of I2a. When combined with published ancient Croatian genomes, the results reveal similar haplogroup diversity and patterns, as well as the expansion of mtDNA haplogroup H over time and a substantial increase in Y-chromosome R1a and I2a haplogroup frequency from the prehistoric to the modern period. Conclusions: Although the analyzed samples are heterogeneous and originate from different historical periods, their genetic signatures conform to the broader patterns expected for the region. In a wider context, the ancient Croatian mitochondrial data reveal stronger genetic persistence from prehistory to modern times, unlike paternal lineages, which show significantly higher divergence. Full article

Influenza-associated encephalitis/encephalopathy (IAE) is a severe neurological complication characterized by central nervous system dysfunction and structural damage following influenza virus infection. Predominantly affecting infants and young children, IAE exhibits its highest incidence in those under five years of age. Key clinical manifestations of IAE include acute seizures, sudden high fever, and impaired consciousness, frequently progressing to coma. Neuroimaging, particularly magnetic resonance imaging (MRI), often reveals multifocal brain lesions involving multiple brain regions, including the cerebellum, brainstem, and corpus callosum. The prognosis of IAE is poor, with a mortality rate reaching 30%. Current diagnosis relies heavily on clinical presentation and characteristic neuroimaging findings, as the precise pathogenesis of IAE remains elusive. While various research models, including cell lines, brain organoids, and animal models, have been developed to recapitulate IAE features, significant limitations persist in modeling the core clinical pathophysiology observed in pediatric patients, necessitating further model refinement. This review synthesizes the clinical spectrum of IAE, summarizes progress in understanding its pathogenesis, and critically evaluates existing research models. We aim to provide a foundation for utilizing experimental approaches to elucidate IAE mechanisms and identify potential therapeutic strategies. Full article

Background/Objectives: This study aimed to evaluate the serum activating transcription factor 4 (ATF4) and toll-like receptor 4 (TLR4) levels in patients with metabolic dysfunction–associated steatotic liver disease (MASLD), and to explain the mechanism in the inflammatory and fibrogenic signaling pathways that are thought to play a role in the development of MASLD through these parameters. Methods: Eighty-eight patients with MASLD and 88 age-sex matched healthy controls were included in this study. Serum ATF4 and TLR4 concentrations were measured using an ELISA method. Results: Both TLR4 (p = 0.010) and ATF4 (p < 0.001) levels were higher in the MASLD group. In this group, TLR4 showed a negative correlation with age. ROC analysis indicated that an ATF4 value of 1.305 or above identified MASLD with 93.2% sensitivity and 85.2% specificity (AUC = 0.968, p < 0.001). For TLR4, a cut-off of 343.5 yielded a sensitivity of 54.5% and a specificity of 70.5% (AUC = 0.613, p = 0.01), indicating limited discriminative ability. Conclusions: Patients with MASLD had higher serum TLR4 and ATF4 levels, consistent with their involvement in inflammatory and fibrotic pathways. ATF4 showed strong diagnostic performance and may serve as a useful non-invasive marker for early MASLD. When evaluated together with TLR4, it may provide complementary information regarding inflammatory pathway activation. Full article

Background/Objectives: Despite large-scale measles and rubella (MR) vaccination campaigns in West Africa, measles outbreaks persist, raising concerns about campaign effectiveness, coverage, and underlying determinants. This study assesses the impact of MR follow-up campaigns in 12 of 17 West African countries (2024–2025) and examines the factors contributing to post-campaign outbreaks. The main objective of this study is to evaluate the impact of MR campaigns on measles transmission, identify the characteristics of post-campaign outbreaks, and propose strategies to improve campaign effectiveness and accelerate progress toward measles elimination in West Africa. Methods: We conducted a cross-sectional and ecological analytical study to examine spatial and temporal variations based on measles surveillance data from 2024 to 2025, post-campaign coverage surveys (PCCS), district-level outbreak reports, and administrative coverage reports. Trends in measles cases before and after the MMR campaigns were assessed, along with demographic characteristics and spatial analyses of confirmed cases. Results: In 2024, 70.5% (12/17) of countries conducted measles vaccination campaigns, but measles outbreaks increased in 2025 (64 districts in 2024 versus 383 in 2025). Children under five remained the most affected (54%), with 85% of cases being either unvaccinated (57%) or of unknown status (28%). Administrative coverage exceeded 95% in most countries, but measles PCCS revealed gaps, with only Senegal (93%) and Guinea-Bissau (94%) achieving high verified coverage. No country achieved 95% national MPCC. Conclusions: Suboptimal campaign quality, gaps in immunity beyond target age groups, and unreliable administrative data contributed to the persistence of outbreaks. Recommendations include extending Measles vaccination campaigns to older children (5–14 years), improving preparedness by drawing on experiences from other programs such as polio, standardizing PCCS data survey and analysis methodologies across all countries, and integrating Measles vaccination campaigns with other services such as nutrition. Full article

Objectives: The Eight-chop technique is a mechanically based nuclear segmentation method designed to improve surgical efficiency and reduce intraocular tissue stress during phacoemulsification. Early postoperative aqueous flare serves as an objective indicator of surgical invasiveness, whereas corneal endothelial cell density (CECD) loss represents a structural measure of endothelial injury. Although both parameters are clinically important, their relationship has not been systematically investigated in the context of this newer mechanical fragmentation approach. Methods: This prospective observational study included 118 eyes from 70 non-diabetic patients undergoing uncomplicated Eight-chop phacoemulsification. Aqueous flare was measured preoperatively and at postoperative Day 1, Day 7, Week 7, and Week 19 using laser flare photometry. CECD was evaluated preoperatively and at Weeks 7 and 19. Changes over time were analyzed using paired t-tests. Linear mixed-effects models (random intercept = patient ID) were constructed to assess predictors of CECD loss and postoperative intraocular pressure (IOP) reduction. Explanatory variables included Day 1 flare, age, preoperative CECD, nucleus hardness (Emery-Little grade), cumulative dissipated energy (CDE), and irrigation fluid volume. Results: Postoperative flare increased significantly at all time points (all p < 0.001), peaking on Day 7 (16.7 ± 9.21 photon counts/ms). CECD loss was extremely small, averaging 1.38% at Week 7 and 1.46% at Week 19. In mixed-effects models, Day 1 flare was not associated with CECD loss at Week 7 (p = 0.35) or Week 19 (p = 0.85). Significant predictors of CECD loss included Emery-Little grade (p = 0.004 at Week 7; p = 0.025 at Week 19), with borderline contributions from CDE and irrigation volume. IOP decreased significantly at Weeks 7 and 19; however, Day 1 flare did not predict IOP reduction. Conclusions: Eight-chop phacoemulsification produced uniformly low postoperative inflammation and exceptionally small corneal endothelial cell loss. Early postoperative flare did not predict CECD loss, suggesting that the Eight-chop technique provides a highly standardized, low-invasiveness surgical environment. These findings suggest that the Eight-chop technique lowers ultrasound energy requirements and may help reduce corneal endothelial stress relative to standard phacoemulsification. Full article

This study aims to explore how industrial heritage regeneration spaces influence employees’ physical and psychological health through restorative perception. With the rapid urbanization and increasing emphasis on sustainable development, the adaptive reuse of industrial heritage sites has become a vital strategy in urban renewal. However, the impact of such spaces on people’s health remains underexplored, especially in terms of how the work environment and restorative psychological mechanisms interact. Using a cross-sectional survey design, data from 486 employees in adaptive reuse projects across major cities in China were analyzed through Structural Equation Modeling (SEM). Employees were chosen as the target population because they represent a group with stable, repeated, and long-term exposure to the regenerated environment during daily routines. Compared with visitors, whose exposure duration, activity purposes, and spatial routes are highly variable, employees provide a more consistent context to test the proposed restorative mechanisms. The results revealed that industrial heritage attribute perception (IHAP), including scale, materiality, historical presence, and functional transformation, significantly predicted restorative perception (β = 0.546, p < 0.001), which in turn positively influenced both psychological health (β = −0.647, p < 0.001) and physical health (β = 0.688, p < 0.001). Instrumental variable analysis using “building age” and “green coverage rate” confirmed the robustness of these findings, showing that restorative perception still significantly improved mental (β = −2.295, p < 0.001) and physical health (β = 0.528, p < 0.001) after addressing endogeneity issues. Furthermore, individual differences such as work tenure (β = 0.239, p < 0.001) and environmental sensitivity (β = 0.054, p > 0.05) moderated these effects. This study extends Attention Restoration Theory (ART) by applying it to historical industrial environments, offering both theoretical insights and practical guidance for designing adaptive reuse spaces that promote employee well-being. Full article

Social media addiction (SMA) and social phobia (SP) are significant adolescent mental health concerns. In Saudi Arabia, despite high social media penetration, the association between these two constructs remains under-researched, particularly in the Aseer region. This cross-sectional study, conducted from January to March 2025, recruited 384 Saudi adolescents aged 11–19 from schools in the Aseer region using multistage cluster sampling. Participants completed validated self-report measures, including the Social Phobia Inventory (SPIN) and the Al-Menayes Social Media Addiction Scale. A refined “Core SMA” subscale was created based on expert consensus criteria to enhance measurement precision. The prevalence of moderate-to-severe social phobia was 15.6%. A significant, moderate positive correlation emerged between SP and SMA (Spearman’s ρ = 0.294, p < 0.001). After adjusting for age, gender, and family income, adolescents with moderate social phobia had 2.15 times the odds of probable SMA compared to those with no SP (adjusted odds ratio [AOR] = 2.15, 95% CI: 1.15–4.04, p < 0.05), and this effect was more pronounced for those with severe social phobia (AOR = 2.56, 95% CI: 1.04–6.30, p < 0.05). This study demonstrates a clear relationship between social phobia severity and social media addiction among Saudi adolescents in the Aseer region. These findings support the urgent need for integrated mental health and digital literacy interventions that proactively screen for both conditions. Full article

Background/Objectives: Frailty is a common geriatric syndrome associated with adverse outcomes such as disability, hospitalization, and mortality. This study aimed to assess the association between body mass index (BMI) trajectories over ten years and frailty among community-dwelling older adults in Brazil. Methods: This population-based longitudinal study used data from the COMO VAI? cohort, conducted with individuals aged ≥60 years in Pelotas, southern Brazil. Frailty was defined in 2024 using Fried’s phenotype, which evaluates weight loss, exhaustion, low physical activity, slowness, and weakness. BMI categories were defined as underweight (BMI < 22.0 kg/m²), eutrophy (22.0–27.0 kg/m²) and overweight (>27.0 kg/m²). BMI trajectories were identified using group-based trajectory modeling for 789 participants with data from at least two of three assessments (2014, 2019, 2024). Only trajectory groups comprising at least 5% of the sample were retained. Associations of baseline BMI and BMI trajectories with frailty were analyzed using Poisson regression with robust variance, adjusted for confounders and calf circumference. Results: Baseline underweight and overweight prevalence were 9.2% and 56.2%, respectively. Trajectory modeling identified three BMI groups: eutrophic (31.6%), overweight (56.4%), and obesity (12.0%). Obesity emerged as a distinct longitudinal trajectory rather than a baseline BMI category. Underweight did not emerge as a distinct BMI trajectory due to its low prevalence over time. Frailty prevalence in 2024 was 36.5%. Overweight trajectory participants had lower frailty prevalence after ten years (PR = 0.73; 95% CI: 0.54–0.99), while baseline underweight was associated with higher frailty ten years later (PR = 1.73; 95% CI: 1.05–2.84), consistent with the known risk of underweight and the potential protective effect observed in overweight older adults. Conclusions: Baseline underweight increased frailty risk, whereas an overweight trajectory showed a potential protective effect, consistent with the “obesity paradox” in older populations. Full article

Background/Objectives: Conservative management of pediatric forearm fractures remains challenging due to the high incidence of secondary displacement. Given the remarkable remodeling potential of children’s bones, clinicians must decide whether to rely on natural healing or intervene. This study evaluated whether accepted secondary displacements affect long-term outcomes and sought to identify predictors of functional impairment. Methods: This retrospective cohort study assessed the long-term outcomes of a cohort of 410 consecutive children who presented with 212 distal metaphyseal and 198 diaphyseal both-bone forearm fractures between 2006–2010. In all patients, closed reduction was recommended for ≥50% displacement, ≥15° angulation (<10 years), or ≥10° angulation (10–16 years). Secondary displacements were frequently accepted, anticipating remodeling. We included 316 children (<16 years) with both-bone forearm fractures (147 diaphyseal, 169 distal metaphyseal), representing 77% of the original cohort, for long-term follow-up (mean 7.2 years, minimum 4 years). Functional and radiographic outcomes were compared between accepted secondary displacements and maintained alignments, stratified by fracture location. Multivariate logistic regression identified predictors of long-term functional impairment, defined as ≥15° loss of pro-supination or QuickDASH ≥ 20. Results: In the distal metaphyseal group there were 50 secondary displacements out of 212 fractures, of which 31 were accepted. In the diaphyseal group there were 60 secondary displacements, of which 49 were accepted. At long-term follow-up, patients with accepted secondary displacements had no clinically relevant differences in functional or radiographic outcomes compared with those with maintained alignments across both diaphyseal and distal metaphyseal fracture groups. For distal fractures, complete initial radial displacements, re-fractures, and bicortical ulnar fractures predicted pro-supination loss ≥ 15° or QuickDASH ≥ 20. For diaphyseal fractures, older age at trauma predicted increased risk of pro-supination limitation. Conclusions: Accepted secondary displacements did not worsen long-term outcomes, supporting reconsideration of strict reduction criteria. The substantial remodeling capacity of pediatric bone—especially in distal metaphyseal fractures in skeletally immature children—should be emphasized when making treatment decisions to avoid unnecessary surgical interventions. Full article

Background. Arm wrestling is a complex strength sport requiring detailed biomechanical analysis. This study investigated elbow functionality in medial epicondylitis using kinematic and electromyographic (EMG) approach. Methods. Hook technique specialists underwent a 10-session rehabilitation program (manual therapy and high-power laser). Outcomes were assessed via the NRS and QuickDASH. Functional analysis utilized surface EMG (Biceps Brachii, Pronator Teres, Brachioradialis, Extensor muscle) and an inertial sensor measuring Mean Jerk (MJ) for movement fluidity. Results. Data analysis for the eleven male athletes (mean age: 22.4 years) revealed substantial improvements following the intervention. NRS decreased from 5.1 to 1.5, and QuickDASH dropped from 25.2 ± 5.3 to 5.5 ± 1.0, while mean jerk remained stable (3.37 to 3.22). Pronator Teres activation markedly increased in the concentric phase (30.14 µV to 127.3 µV), indicating better coordination. Biceps Brachii (BB): Assumed a more pronounced concentric role, likely a compensatory adaptation after pain reduction; and lastly, Common Finger Extensor increased activation suggested increased extensor loading during the push phase. Conclusions. The combined kinematic and EMG data were crucial for identifying underlying musculoskeletal dysfunctions. The findings support an integrated approach for elbow health in arm wrestlers, providing objective data for targeted rehabilitation and prevention programs focusing on both pain and neuromuscular coordination. Full article