Double-Stranded RNAs (dsRNAs) as a Sustainable Tool against Gray Mold (Botrytis cinerea) in Grapevine: Effectiveness of Different Application Methods in an Open-Air Environment

Nerva, Luca; Sandrini, Marco; Gambino, Giorgio; Chitarra, Walter

doi:10.3390/biom10020200

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Double-Stranded RNAs (dsRNAs) as a Sustainable Tool against Gray Mold (Botrytis cinerea) in Grapevine: Effectiveness of Different Application Methods in an Open-Air Environment

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Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano, Italy

Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino, Italy

Dipartimento di Scienze Agroalimentari, Ambientali e Animali, Università degli Studi di Udine, Via delle Scienze 206, 33100 Udine, Italy

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Biomolecules 2020, 10(2), 200; https://doi.org/10.3390/biom10020200

Received: 22 December 2019 / Revised: 12 January 2020 / Accepted: 27 January 2020 / Published: 29 January 2020

(This article belongs to the Section Natural and Bio-inspired Molecules)

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Abstract

Grapevine is one of the most important and globally widespread fruit species, with a high impact on the economy of many countries but with an intense environmental effect. Therefore, new environmentally friendly defense strategies against fungal pathogens are needed for more sustainable agriculture. A novel emerging approach is spray-induced gene silencing (SIGS), which concerns the exogenous application of double-stranded RNA (dsRNA) inducing enhanced plant resistance against fungal pathogens. Here, we tested the ability of SIGS to prevent and counteract infection of Botrytis cinerea, one of the most economically impacting pathogens of grapevine. In particular, we tested three independent approaches for dsRNA delivery into plants: (i) high pressure spraying of leaves; (ii) petiole adsorption of dsRNAs; (iii) postharvest spraying of bunches. We demonstrated that independently from the method of application, SIGS can reduce virulence of the fungus. Moreover, we also observed three different levels of efficacy depending on the method of application. Thus, the present data provide crucial information on the possibility to exploit SIGS as an alternative sustainable and ecofriendly strategy for grapevine pre- and postharvest protection. View Full-Text

Keywords: dsRNA; spray induced gene silencing; plant protection; sustainability; grapevine; Vitis vinifera; Botrytis cinerea

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Nerva, L.; Sandrini, M.; Gambino, G.; Chitarra, W. Double-Stranded RNAs (dsRNAs) as a Sustainable Tool against Gray Mold (Botrytis cinerea) in Grapevine: Effectiveness of Different Application Methods in an Open-Air Environment. Biomolecules 2020, 10, 200. https://doi.org/10.3390/biom10020200

AMA Style

Nerva L, Sandrini M, Gambino G, Chitarra W. Double-Stranded RNAs (dsRNAs) as a Sustainable Tool against Gray Mold (Botrytis cinerea) in Grapevine: Effectiveness of Different Application Methods in an Open-Air Environment. Biomolecules. 2020; 10(2):200. https://doi.org/10.3390/biom10020200

Chicago/Turabian Style

Nerva, Luca, Marco Sandrini, Giorgio Gambino, and Walter Chitarra. 2020. "Double-Stranded RNAs (dsRNAs) as a Sustainable Tool against Gray Mold (Botrytis cinerea) in Grapevine: Effectiveness of Different Application Methods in an Open-Air Environment" Biomolecules 10, no. 2: 200. https://doi.org/10.3390/biom10020200

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Double-Stranded RNAs (dsRNAs) as a Sustainable Tool against Gray Mold (Botrytis cinerea) in Grapevine: Effectiveness of Different Application Methods in an Open-Air Environment

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