Erinacine C Activates Transcription from a Consensus ETS DNA Binding Site in Astrocytic Cells in Addition to NGF Induction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plasmid Construction
2.2. Cell Culture
2.3. Pharmacological Studies
2.4. PC12 Cell Differentiation Analysis
2.5. Transfection
2.6. Luciferase Assays
2.7. Semiquantitative RT-PCR
2.8. Origin of Erinacine C
2.9. Statistical Analysis
3. Results
3.1. Erinacine C Induces Expression of Nerve Growth Factor (NGF) in Astrocytoma Cells
3.2. Erinacine C Conditioned Astrocytoma Medium Likely Acts via Known TrkA-Mediated Signaling Pathway
3.3. Transcription Factor Mediated Signal Transduction Activated by Erinacine C
3.4. Erinacine C Activates ETS-Dependent Transcription
3.5. Erinacine C is Unlikely to be Sufficient to Induce ngf Transcription in 1321N1 Astrocytic Cells
3.6. Erinacine C Induced ETS Activity May Occur Independently of ngf and bdnf Induction
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Rascher, M.; Wittstein, K.; Winter, B.; Rupcic, Z.; Wolf-Asseburg, A.; Stadler, M.; Köster, R.W. Erinacine C Activates Transcription from a Consensus ETS DNA Binding Site in Astrocytic Cells in Addition to NGF Induction. Biomolecules 2020, 10, 1440. https://doi.org/10.3390/biom10101440
Rascher M, Wittstein K, Winter B, Rupcic Z, Wolf-Asseburg A, Stadler M, Köster RW. Erinacine C Activates Transcription from a Consensus ETS DNA Binding Site in Astrocytic Cells in Addition to NGF Induction. Biomolecules. 2020; 10(10):1440. https://doi.org/10.3390/biom10101440
Chicago/Turabian StyleRascher, Monique, Kathrin Wittstein, Barbara Winter, Zeljka Rupcic, Alexandra Wolf-Asseburg, Marc Stadler, and Reinhard W. Köster. 2020. "Erinacine C Activates Transcription from a Consensus ETS DNA Binding Site in Astrocytic Cells in Addition to NGF Induction" Biomolecules 10, no. 10: 1440. https://doi.org/10.3390/biom10101440