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Open AccessArticle

Production of Active Poly- and Oligosaccharidic Fractions from Ulva sp. by Combining Enzyme-Assisted Extraction (EAE) and Depolymerization

1
Université Catholique de l’Ouest Bretagne Nord, 22200 Guingamp, France
2
Laboratoire de Biotechnologie et Chimie Marines, EA 3884 Université Bretagne Sud, 56000 Vannes, France
*
Author to whom correspondence should be addressed.
Metabolites 2019, 9(9), 182; https://doi.org/10.3390/metabo9090182
Received: 19 July 2019 / Revised: 8 September 2019 / Accepted: 10 September 2019 / Published: 12 September 2019
(This article belongs to the Special Issue Seaweeds Metabolites)
Data on fractionation and depolymerization of the matrix ulvan polysaccharides, and studies on the biological activities on skin cells, are very scarce. In this work, crude ulvans were produced by using EAE (enzyme-assisted extraction) and compared to maceration (an established procedure). After different fractionation procedures—ethanolic precipitation, dialysis, or ammonium sulfate precipitation—the biochemical composition showed that EAE led to an increased content in ulvans. Coupling EAE to sulfate ammonium precipitation led to protein enrichment. Oligosaccharides were obtained by using radical depolymerization by H2O2 and ion-exchange resin depolymerization. Sulfate groups were partially cleaved during these chemical treatments. The potential bioactivity of the fractions was assessed using a lipoxygenase inhibition assay for anti-inflammatory activity and a WST-1 assay for human dermal fibroblast viability and proliferation. All ulvans extracts, poly- and oligosaccharidic fractions from EAE, expanded the fibroblast proliferation rate up to 62%. Our research emphasizes the potential use of poly- and oligosaccharidic fractions of Ulva sp. for further development in cosmetic applications. View Full-Text
Keywords: green seaweed; polysaccharide; ulvan; enzyme-assisted extraction; endo-protease Protamex®; depolymerization; human dermal fibroblast; lipoxygenase green seaweed; polysaccharide; ulvan; enzyme-assisted extraction; endo-protease Protamex®; depolymerization; human dermal fibroblast; lipoxygenase
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Fournière, M.; Latire, T.; Lang, M.; Terme, N.; Bourgougnon, N.; Bedoux, G. Production of Active Poly- and Oligosaccharidic Fractions from Ulva sp. by Combining Enzyme-Assisted Extraction (EAE) and Depolymerization. Metabolites 2019, 9, 182.

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