An Isotopic Ratio Outlier Analysis Approach for Global Metabolomics of Biosynthetically Talented Actinomycetes
Abstract
:1. Introduction
2. Results
2.1. IROA Approach Enabling Detection of Metabolites Whose Production Is Initiated or Terminated in Response to Experimental Treatment
2.2. N. dassonvillei Metabolome Revealed by Untargeted IROA UHPLC/MS
2.3. IROA LC/MS Differentiates Treatment and Control N. dassonvillei Metabolomes and Suggests the Production of Novel Metabolites
2.4. Impacts of Bipyridyl Treatment on N. dassonvillei Biomass and Siderophore Production
2.5. IROA Enables Rapid Assessment of the Perturbation of Targeted Metabolic Pathways
3. Discussion
4. Materials and Methods
4.1. Nocardiopsis dassonvillei Cultures and Fermentation for IROA Experiments
4.2. Chemical Extraction and Preparation of Samples for UHPLC/MS Metabolomics
4.3. UHPLC/MS Analysis
4.4. Data Processing and Analyses
4.5. Evaluation of Biomass and Siderophore Production
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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ESI Mode | |||
---|---|---|---|
Positive | Negative | Total | |
IROA peak pairs detected | 1243 | 89 | 1332 |
Identified metabolites | 85 | 22 | 107 |
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Carey, J.; Nguyen, T.; Korchak, J.; Beecher, C.; de Jong, F.; Lane, A.L. An Isotopic Ratio Outlier Analysis Approach for Global Metabolomics of Biosynthetically Talented Actinomycetes. Metabolites 2019, 9, 181. https://doi.org/10.3390/metabo9090181
Carey J, Nguyen T, Korchak J, Beecher C, de Jong F, Lane AL. An Isotopic Ratio Outlier Analysis Approach for Global Metabolomics of Biosynthetically Talented Actinomycetes. Metabolites. 2019; 9(9):181. https://doi.org/10.3390/metabo9090181
Chicago/Turabian StyleCarey, Jordan, Thanh Nguyen, Jennifer Korchak, Christopher Beecher, Felice de Jong, and Amy L. Lane. 2019. "An Isotopic Ratio Outlier Analysis Approach for Global Metabolomics of Biosynthetically Talented Actinomycetes" Metabolites 9, no. 9: 181. https://doi.org/10.3390/metabo9090181