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Surfactant Lipidomics of Alveolar Lavage Fluid in Mice Based on Ultra-High-Performance Liquid Chromatography Coupled to Hybrid Quadrupole-Exactive Orbitrap Mass Spectrometry

by Rui Yang 1,2,3, Ying Zhang 4, Wenjuan Qian 1,2, Linxiu Peng 2,3, Lili Lin 2,3, Jia Xu 2, Tong Xie 2,3, Jianjian Ji 3, Xiuqin Zhan 1,* and Jinjun Shan 1,2,3,*
1
Jiangsu Key Laboratory of Pediatric Respiratory Disease, School of Medicine and Life Sciences, Nanjing University of Chinese Medicine, Nanjing 210023, China
2
Medical Metabolomics Center, Nanjing University of Chinese Medicine, Nanjing 210023, China
3
Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China
4
Genome Center of UC Davis, NIH West Coast Metabolomics Center, Davis, CA 95616, USA
*
Authors to whom correspondence should be addressed.
Metabolites 2019, 9(4), 80; https://doi.org/10.3390/metabo9040080
Received: 20 December 2018 / Revised: 18 April 2019 / Accepted: 23 April 2019 / Published: 25 April 2019
(This article belongs to the Special Issue Compound Identification of Small Molecules)
Surfactant lipid metabolism is closely related to pulmonary diseases. Lipid metabolism disorder can cause lung diseases, vice versa. With this rationale, a useful method was established in this study to determine the lipidome in bronchoalveolar lavage fluid (BALF) of mice. The lipid components in BALF were extracted by liquid–liquid extraction (methanol and methyl tert-butyl ether, and water). Ultra-high-performance liquid chromatography coupled to hybrid Quadrupole-Exactive Orbitrap mass spectrometry was used to analyze the extracted samples, which showed a broad scanning range of 215–1800 m/z. With MS-DIAL software and built-in LipidBlast database, we identified 38 lipids in positive, and 31 lipids in negative, ion mode, including lysophosphatidylcholine (lysoPC), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), etc. Then, the changes of lipids in BALF of mice with acute lung injury (ALI) induced by lipopolysaccharide (LPS) was investigated, which may contribute to further exploration of the pathogenesis of ALI. View Full-Text
Keywords: surfactant lipidomics; high-resolution mass spectrometry; acute lung injury; bronchoalveolar lavage fluid; lipopolysaccharide surfactant lipidomics; high-resolution mass spectrometry; acute lung injury; bronchoalveolar lavage fluid; lipopolysaccharide
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Yang, R.; Zhang, Y.; Qian, W.; Peng, L.; Lin, L.; Xu, J.; Xie, T.; Ji, J.; Zhan, X.; Shan, J. Surfactant Lipidomics of Alveolar Lavage Fluid in Mice Based on Ultra-High-Performance Liquid Chromatography Coupled to Hybrid Quadrupole-Exactive Orbitrap Mass Spectrometry. Metabolites 2019, 9, 80.

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