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Metabolites 2017, 7(4), 53; https://doi.org/10.3390/metabo7040053

Analysis of Intracellular Metabolites from Microorganisms: Quenching and Extraction Protocols

1
The New Zealand Institute for Plant & Food Research Limited, Private Bag 92169, Auckland 1142, New Zealand
2
School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1010, New Zealand
3
Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Crown Street, Liverpool L693BX, UK
*
Author to whom correspondence should be addressed.
Received: 1 September 2017 / Revised: 11 October 2017 / Accepted: 21 October 2017 / Published: 23 October 2017
(This article belongs to the Special Issue Microbial Metabolomics Volume 2)
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Abstract

Sample preparation is one of the most important steps in metabolome analysis. The challenges of determining microbial metabolome have been well discussed within the research community and many improvements have already been achieved in last decade. The analysis of intracellular metabolites is particularly challenging. Environmental perturbations may considerably affect microbial metabolism, which results in intracellular metabolites being rapidly degraded or metabolized by enzymatic reactions. Therefore, quenching or the complete stop of cell metabolism is a pre-requisite for accurate intracellular metabolite analysis. After quenching, metabolites need to be extracted from the intracellular compartment. The choice of the most suitable metabolite extraction method/s is another crucial step. The literature indicates that specific classes of metabolites are better extracted by different extraction protocols. In this review, we discuss the technical aspects and advancements of quenching and extraction of intracellular metabolite analysis from microbial cells. View Full-Text
Keywords: metabolite footprinting; exometabolome; culture medium; metabolic modelling; sample preparation; analytical instruments; data integration; extracellular metabolites; intracellular metabolites metabolite footprinting; exometabolome; culture medium; metabolic modelling; sample preparation; analytical instruments; data integration; extracellular metabolites; intracellular metabolites
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Pinu, F.R.; Villas-Boas, S.G.; Aggio, R. Analysis of Intracellular Metabolites from Microorganisms: Quenching and Extraction Protocols. Metabolites 2017, 7, 53.

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