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Metabolites 2017, 7(3), 43; https://doi.org/10.3390/metabo7030043

Extracellular Microbial Metabolomics: The State of the Art

1
The New Zealand Institute for Plant & Food Research Limited, Private Bag 92169, Auckland 1142, New Zealand
2
School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1010, New Zealand
*
Author to whom correspondence should be addressed.
Received: 31 July 2017 / Revised: 21 August 2017 / Accepted: 22 August 2017 / Published: 22 August 2017
(This article belongs to the Special Issue Microbial Metabolomics Volume 2)
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Abstract

Microorganisms produce and secrete many primary and secondary metabolites to the surrounding environment during their growth. Therefore, extracellular metabolites provide important information about the changes in microbial metabolism due to different environmental cues. The determination of these metabolites is also comparatively easier than the extraction and analysis of intracellular metabolites as there is no need for cell rupture. Many analytical methods are already available and have been used for the analysis of extracellular metabolites from microorganisms over the last two decades. Here, we review the applications and benefits of extracellular metabolite analysis. We also discuss different sample preparation protocols available in the literature for both types (e.g., metabolites in solution and in gas) of extracellular microbial metabolites. Lastly, we evaluate the authenticity of using extracellular metabolomics data in the metabolic modelling of different industrially important microorganisms. View Full-Text
Keywords: metabolite footprinting; exometabolome; culture medium; metabolic modelling; sample preparation; analytical instruments; data integration; extracellular metabolites; intracellular metabolites metabolite footprinting; exometabolome; culture medium; metabolic modelling; sample preparation; analytical instruments; data integration; extracellular metabolites; intracellular metabolites
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Pinu, F.R.; Villas-Boas, S.G. Extracellular Microbial Metabolomics: The State of the Art. Metabolites 2017, 7, 43.

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