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Open AccessArticle

A Novel Solid Phase Extraction Sample Preparation Method for Lipidomic Analysis of Human Plasma Using Liquid Chromatography/Mass Spectrometry

1
Agilent Technologies, Santa Clara, CA 95051, USA
2
Agilent Technologies, Wilmington, DE 19808, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Leonardo Tenori
Metabolites 2021, 11(5), 294; https://doi.org/10.3390/metabo11050294
Received: 19 March 2021 / Revised: 27 April 2021 / Accepted: 28 April 2021 / Published: 4 May 2021
(This article belongs to the Special Issue Mass Spectrometry-Based Lipidomics)
Lipidomic approaches are widely used to investigate the relationship between lipids, human health, and disease. Conventional sample preparation techniques for the extraction of lipids from biological matrices like human plasma are based on liquid-liquid extraction (LLE). However, these methods are labor-intensive, time-consuming, and can show poor reproducibility and selectivity on lipid extraction. A novel, solid-phase extraction (SPE) approach was demonstrated to extract lipids from human plasma using a lipid extraction SPE in both cartridge and 96-well-plate formats, followed by analysis using a combination of targeted and untargeted liquid chromatography/mass spectrometry. The Lipid Extraction SPE method was compared to traditional LLE methods for lipid class recovery, lipidome coverage, and reproducibility. The novel SPE method used a simplified protocol with significant time and labor savings and provided equivalent or better qualitative and quantitative results than traditional LLE methods with respect to several critical performance metrics; recovery, reproducibility, and lipidome coverage. View Full-Text
Keywords: lipidomics; lipids; sample preparation; SPE; mass spectrometry; liquid chromatography lipidomics; lipids; sample preparation; SPE; mass spectrometry; liquid chromatography
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MDPI and ACS Style

Apffel, A.; Zhao, L.; Sartain, M.J. A Novel Solid Phase Extraction Sample Preparation Method for Lipidomic Analysis of Human Plasma Using Liquid Chromatography/Mass Spectrometry. Metabolites 2021, 11, 294. https://doi.org/10.3390/metabo11050294

AMA Style

Apffel A, Zhao L, Sartain MJ. A Novel Solid Phase Extraction Sample Preparation Method for Lipidomic Analysis of Human Plasma Using Liquid Chromatography/Mass Spectrometry. Metabolites. 2021; 11(5):294. https://doi.org/10.3390/metabo11050294

Chicago/Turabian Style

Apffel, Alex; Zhao, Limian; Sartain, Mark J. 2021. "A Novel Solid Phase Extraction Sample Preparation Method for Lipidomic Analysis of Human Plasma Using Liquid Chromatography/Mass Spectrometry" Metabolites 11, no. 5: 294. https://doi.org/10.3390/metabo11050294

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