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Metabolites
Volume 10
Issue 6
10.3390/metabo10060233
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Article

The Bovine Metabolome

by
Aidin Foroutan
1,2,
Carolyn Fitzsimmons
1,3,
Rupasri Mandal
2,
Hamed Piri-Moghadam
2,
Jiamin Zheng
2,
AnChi Guo
2,
Carin Li
2,
Le Luo Guan
1 and
David S. Wishart
2,4,*
1
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada
2
Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada
3
Agriculture and Agri-Food Canada, Edmonton, AB T6G 2P5, Canada
4
Department of Computing Science, University of Alberta, Edmonton, AB T6G 2E8, Canada
*
Author to whom correspondence should be addressed.
Metabolites 2020, 10(6), 233; https://doi.org/10.3390/metabo10060233
Submission received: 8 May 2020 / Revised: 1 June 2020 / Accepted: 2 June 2020 / Published: 5 June 2020
(This article belongs to the Section Animal Metabolism)
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Abstract

From an animal health perspective, relatively little is known about the typical or healthy ranges of concentrations for many metabolites in bovine biofluids and tissues. Here, we describe the results of a comprehensive, quantitative metabolomic characterization of six bovine biofluids and tissues, including serum, ruminal fluid, liver, Longissimus thoracis (LT) muscle, semimembranosus (SM) muscle, and testis tissues. Using nuclear magnetic resonance (NMR) spectroscopy, liquid chromatography–tandem mass spectrometry (LC–MS/MS), and inductively coupled plasma–mass spectrometry (ICP–MS), we were able to identify and quantify more than 145 metabolites in each of these biofluids/tissues. Combining these results with previous work done by our team on other bovine biofluids, as well as previously published literature values for other bovine tissues and biofluids, we were able to generate quantitative reference concentration data for 2100 unique metabolites across five different bovine biofluids and seven different tissues. These experimental data were combined with computer-aided, genome-scale metabolite inference techniques to add another 48,628 unique metabolites that are biochemically expected to be in bovine tissues or biofluids. Altogether, 51,801 unique metabolites were identified in this study. Detailed information on these 51,801 unique metabolites has been placed in a publicly available database called the Bovine Metabolome Database.
Keywords: bovine; tissue; biofluid; NMR; LC–MS/MS; ICP–MS; metabolite bovine; tissue; biofluid; NMR; LC–MS/MS; ICP–MS; metabolite
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MDPI and ACS Style

Foroutan, A.; Fitzsimmons, C.; Mandal, R.; Piri-Moghadam, H.; Zheng, J.; Guo, A.; Li, C.; Guan, L.L.; Wishart, D.S. The Bovine Metabolome. Metabolites 2020, 10, 233. https://doi.org/10.3390/metabo10060233

AMA Style

Foroutan A, Fitzsimmons C, Mandal R, Piri-Moghadam H, Zheng J, Guo A, Li C, Guan LL, Wishart DS. The Bovine Metabolome. Metabolites. 2020; 10(6):233. https://doi.org/10.3390/metabo10060233

Chicago/Turabian Style

Foroutan, Aidin, Carolyn Fitzsimmons, Rupasri Mandal, Hamed Piri-Moghadam, Jiamin Zheng, AnChi Guo, Carin Li, Le Luo Guan, and David S. Wishart. 2020. "The Bovine Metabolome" Metabolites 10, no. 6: 233. https://doi.org/10.3390/metabo10060233

APA Style

Foroutan, A., Fitzsimmons, C., Mandal, R., Piri-Moghadam, H., Zheng, J., Guo, A., Li, C., Guan, L. L., & Wishart, D. S. (2020). The Bovine Metabolome. Metabolites, 10(6), 233. https://doi.org/10.3390/metabo10060233

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