Next Article in Journal
Recent Advances on Topical Application of Ceramides to Restore Barrier Function of Skin
Next Article in Special Issue
Natural and Bioinspired Phenolic Compounds as Tyrosinase Inhibitors for the Treatment of Skin Hyperpigmentation: Recent Advances
Previous Article in Journal
The Influence of Facial Muscle Training on the Facial Soft Tissue Profile: A Brief Review
Previous Article in Special Issue
Use of Vegetable Oils to Improve the Sun Protection Factor of Sunscreen Formulations
Open AccessCommunication

Calebin-A, a Curcuminoid Analog Inhibits α-MSH-Induced Melanogenesis in B16F10 Mouse Melanoma Cells

1
Department of Biomedical Engineering; Stony Brook University, Stony Brook, NY 11794, USA
2
Sabinsa Corporation, 20 Lake Drive, East Windsor, NJ 08520, USA
3
Department of Pathology, Stony Brook University, Stony Brook, NY 11794, USA
4
Department of Biochemistry and Cellular Biology, Stony Brook University, Stony Brook, NY, 11794, USA
*
Author to whom correspondence should be addressed.
Cosmetics 2019, 6(3), 51; https://doi.org/10.3390/cosmetics6030051
Received: 26 July 2019 / Revised: 13 August 2019 / Accepted: 15 August 2019 / Published: 19 August 2019
(This article belongs to the Special Issue Melanogenesis and Melanin-Related Compounds: A Cosmetic Perspective)
Hyperpigmentation skin disorders comprise melasma, age spots, and post-inflammatory hyperpigmentation. They are characterized by an aberrant upregulation of melanin pigment and pose a significant burden aesthetically. Calebin-A (CBA) is a natural curcuminoid analog derived from turmeric root (Curcuma longa) but, unlike curcumin, it has not been explored yet for anti-melanogenic activity. Hence, in the current study, we studied CBA for its effects on α-melanocyte stimulating hormone (αMSH)-stimulated melanogenesis in B16F10 mouse melanoma cells. Our results showed that CBA (20 μM) significantly suppressed αMSH-stimulated melanogenesis after 48 h treatment. The underlying mechanisms of CBA’s anti-melanogenic activity were studied, and it was shown that CBA did not affect either intracellular tyrosinase activity or the direct activity of tyrosinase enzyme. Additionally, CBA did not affect intracellular α-glucosidase activity but significantly inhibited direct α-glucosidase activity. CBA also directly scavenged 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radicals, consistent with potent antioxidant activity but did not inhibit intracellular reactive oxygen species (ROS). CBA increased acidification of cellular organelles and inhibited maturation of melanosomes by significantly reducing the number of mature melanosomes. Our results indicate that CBA may hold promise as a pigmentation inhibitor for hyperpigmentation disorders for cosmetic use by targeting pathways other than tyrosinase inhibition. Further studies to delineate the molecular signaling mechanism of melanogenesis inhibition and test anti-melanogenesis efficacy of CBA in human skin melanocytes and skin equivalents are warranted. View Full-Text
Keywords: Calebin-A; melanogenesis; α-MSH; organelles acidification; melanosome maturation Calebin-A; melanogenesis; α-MSH; organelles acidification; melanosome maturation
Show Figures

Figure 1

MDPI and ACS Style

Goenka, S.; Nagabhushanam, K.; Majeed, M.; Simon, S.R. Calebin-A, a Curcuminoid Analog Inhibits α-MSH-Induced Melanogenesis in B16F10 Mouse Melanoma Cells. Cosmetics 2019, 6, 51.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop