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Cosmetics
Volume 12
Issue 6
10.3390/cosmetics12060279
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Ex Vivo Human Skin as a Platform to Study Cosmetic Modulation of Specialized Pro-Resolving Mediators

by
Michele Massironi
1,
Lorenzo Zanella
2,*,
Francesca Benato
1,
Camila Paz Quezada Meza
1,
Chiara Rompietti
1,
Sandro Rosa
1,
Dominik Stuhlmann
3,
Martina Herrmann
3 and
Marco Massironi
1,*
1
Symrise Srl, 35129 Padova, Italy
2
Independent Researcher, 30173 Venezia, Italy
3
Symrise AG, 37603 Holzminden, Germany
*
Authors to whom correspondence should be addressed.
Cosmetics 2025, 12(6), 279; https://doi.org/10.3390/cosmetics12060279 (registering DOI)
Submission received: 16 October 2025 / Revised: 21 November 2025 / Accepted: 6 December 2025 / Published: 10 December 2025
(This article belongs to the Section Cosmetic Dermatology)
Versions Notes

Abstract

Chronic low-grade inflammation and oxidative stress induced by the exposome represent key drivers of skin aging and related imperfections. The development of experimental models suitable for studying these metabolic processes is therefore of primary importance for the cosmetic industry. In recent years, the role of specialized pro-resolving mediators (SPMs) in the resolution of inflammation has been highlighted; however, in vitro skin models to investigate them are still lacking. In this work, we developed an ex vivo human skin culture model that allows the quantification of maresin 1 (MaR1) production by measuring its concentration in the conditioned culture medium using an ELISA-based assay. The presence and survival of MaR1-synthesizing immune cells, namely Langerhans cells and leukocytes, were quantified during the first days of culture. The model’s ability to modulate MaR1 production was assessed in response to treatment with its precursor, docosahexaenoic acid (DHA), and with a DHA-rich cosmetic ingredient named Isochrysis Galbana Extract. Results demonstrated that the model produces MaR1 even in the absence of stimulation and responds to treatments with a further increase in MaR1 production. Furthermore, the tissue-to-medium ratio required to obtain MaR1 concentrations suitable for effective ELISA quantification was optimized. This model establishes a reproducible and scalable experimental platform for quantifying SPMs and evaluating DHA-based formulations, supporting both cosmetic research and mechanistic investigations.
Keywords: Maresin 1; resolvins; skin immunity; specialized pro-resolving mediators (SPMs); Langerhans cells; inflammation Maresin 1; resolvins; skin immunity; specialized pro-resolving mediators (SPMs); Langerhans cells; inflammation

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MDPI and ACS Style

Massironi, M.; Zanella, L.; Benato, F.; Quezada Meza, C.P.; Rompietti, C.; Rosa, S.; Stuhlmann, D.; Herrmann, M.; Massironi, M. Ex Vivo Human Skin as a Platform to Study Cosmetic Modulation of Specialized Pro-Resolving Mediators. Cosmetics 2025, 12, 279. https://doi.org/10.3390/cosmetics12060279

AMA Style

Massironi M, Zanella L, Benato F, Quezada Meza CP, Rompietti C, Rosa S, Stuhlmann D, Herrmann M, Massironi M. Ex Vivo Human Skin as a Platform to Study Cosmetic Modulation of Specialized Pro-Resolving Mediators. Cosmetics. 2025; 12(6):279. https://doi.org/10.3390/cosmetics12060279

Chicago/Turabian Style

Massironi, Michele, Lorenzo Zanella, Francesca Benato, Camila Paz Quezada Meza, Chiara Rompietti, Sandro Rosa, Dominik Stuhlmann, Martina Herrmann, and Marco Massironi. 2025. "Ex Vivo Human Skin as a Platform to Study Cosmetic Modulation of Specialized Pro-Resolving Mediators" Cosmetics 12, no. 6: 279. https://doi.org/10.3390/cosmetics12060279

APA Style

Massironi, M., Zanella, L., Benato, F., Quezada Meza, C. P., Rompietti, C., Rosa, S., Stuhlmann, D., Herrmann, M., & Massironi, M. (2025). Ex Vivo Human Skin as a Platform to Study Cosmetic Modulation of Specialized Pro-Resolving Mediators. Cosmetics, 12(6), 279. https://doi.org/10.3390/cosmetics12060279

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