Characterisation of NiTi Orthodontic Archwires Surface after the Simulation of Mechanical Loading in CACO2-2 Cell Culture
, Branislav Glišić 1, Monika Jenko 2, Matjaž Godec 2, Samo Hočevar 3 and Rebeka Rudolf 4,*
Round 1
Reviewer 1 Report
The work consist only in one experimental test with particular set up, more kind of tested orthodontic wires would be improve the scientific soundness, giving a useful comparison. But the work is conducted in precise way and the evaluation of the effect of mechanical stress it is a good vision of the problem of Ni release. Therefore if the authors don't prefer to amplify their work, I think only this is acceptable for publication, maybe as a first step which can develop in a wider experimental series of test. I suggest only:
increase the size of Fig.1(b) to allows a better vision of the experimental set up
line 219: concentracion instead concentration
please use the same concentration scale for Fig 5 (a) and (b)
Author Response
The authors would like to thank again the reviewers for their quick review process and for the acceptance of the article. All the issues raised by the reviewers were addressed and modifications are made to the paper as per the reviewers’ guidelines. The changes are listed below:
Reviewer 1:
increase the size of Fig.1(b) to allows a better vision of the experimental set up - done
line 219: concentracion instead concentration – done
please use the same concentration scale for Fig 5 (a) and (b) – done
With respect in the name of all authors.
Author Response File: 
Author Response.docx
Reviewer 2 Report
This is an interesting work on the characterization of NiTi Orthodontic Archwires surface after mechanical loading. Some criticism are present:
1. Lines 17-27 the abstract is poorly written. In particular some statements must be added in the initial part about research question and problems
2. Line 86 The choice of CACO2-2 cell culture can be discussed. In particular, assessing the potential precence of Ni on the exposed cells is not explained in terms of biocompatibility. Some considerations on the possible cytotoxic effects of Ni on cells must be provided.
3. Furthermore, it is not clear why keratinocytes were not used; the authors must give explanations.
4. Line 90 Can the choice of 24h be better explained?
5. Starting froma scientific work of 1999, we hope not to consider more innovative aspects in this regard. In particular, to add a proof of immediate release (1h, 12h) would be highly desiderable . I this regard i advise the authors to insert in the reference sction and discuss the following manuscript:
-Pagano S, Chieruzzi M, Balloni S, Lombardo G, Torre L, Bodo M, Cianetti S, MAirnucci L Biological, thermal and mechanical characterization of modified dlass ionomer cements:the role of nanohydroxyapatite, ciprofloxacin and Zinc- l Carnosine. MAter Sci Eng C Mater Biol Appl.2019 Jan 1;94:76-85
6. Some consideration must be added on the possible effect of NiTi respect to traditional orthodontic archwires on mechanical loading on natural and or treated teeth. In this context the authors must added this scientific paper:
-Chieruzzi M, PAgano S, Cianetti S, Lombardo G, Kenny JM, Torre L Effect of fibre post, bone losses and fibre content on the biomechanical behaviour of endodontically treated teeth: 3D-finite alement analysis. MAter Sci Eng C Mater Bio Appl. 2017 MAy 1;74:334-346
7. Line 100: the end of the introduction seciton shoul be structured as follow: "The aim of this work......."Moreover null hypotheses are not present
8. Line 137: Name, model company and Nation of Instruments must be added
9. Line 259 Some important considerations must be added about the possible role of oral fluids on the surface variations of orthodontic attacks.. Why have no tests been provided for this?
10. The bibliography is very old and i advise the authors to insert some vewer works, critically explaining them in the text.
11. About treatment chances:
-D'Attilio M, Rodolfino D, Filippakos A, Saccucci M, Festa F, Tripodi D Second class resolver: a retrospective analysis Eur J Paedriatic Dent 2014; 15(1):78-82
-Baldini A, Nota A, Santariello C, Assi V, Ballanti F, Gatto R, Cozza P Sagittal dentoskeletal modifications associated with different activation protocols of rapid maxillary expansion Eur J Paedriatic Dent 2018;19(2):151-155
Author Response
The authors would like to thank again the reviewers for their quick review process and for the acceptance of the article. All the issues raised by the reviewers were addressed and modifications are made to the paper as per the reviewers’ guidelines. The changes are listed below:
Reviewer 2:
1. Lines 17-27 the abstract is poorly written
Abstract changed. Added Lines 18-23.
2. Line 86 The choice of CACO2-2 cell culture can be discussed. In particular, assessing the potential precence of Ni on the exposed cells is not explained in terms of biocompatibility.
Added text Lines 100-105
3. Furthermore, it is not clear why keratinocytes were not used; the authors must give explanations.
Lines 112- 118
Keratinocytes were not readily available during the preparation of this research.
4. Line 90 Can the choice of 24h be better explained?
Line 100-105
5. Starting froma scientific work of 1999, we hope not to consider more innovative aspects in this regard. In particular, to add a proof of immediate release (1h, 12h) would be highly desiderable . I this regard i advise the authors to insert in the reference sction and discuss the following manuscript: -Pagano S, Chieruzzi M, Balloni S, Lombardo G, Torre L, Bodo M, Cianetti S, MAirnucci L Biological, thermal and mechanical characterization of modified dlass ionomer cements:the role of nanohydroxyapatite, ciprofloxacin and Zinc- l Carnosine. MAter Sci Eng C Mater Biol Appl.2019 Jan 1;94:76-85
112-118
6. Some consideration must be added on the possible effect of NiTi respect to traditional orthodontic archwires on mechanical loading on natural and or treated teeth. In this context the authors must added this scientific paper:
-Chieruzzi M, PAgano S, Cianetti S, Lombardo G, Kenny JM, Torre L Effect of fibre post, bone losses and fibre content on the biomechanical behaviour of endodontically treated teeth: 3D-finite alement analysis. MAter Sci Eng C Mater Bio Appl. 2017 MAy 1;74:334-346
Lines 83-86
7. Line 100: the end of the introduction seciton shoul be structured as follow: "The aim of this work......."Moreover null hypotheses are not present
119-124
8 . Line 137: Name, model company and Nation of Instruments must be added
Line 165-166
9. Line 259 Some important considerations must be added about the possible role of oral fluids on the surface variations of orthodontic attacks.. Why have no tests been provided for this?
Lines 286-288, 306-308
10. The bibliography is very old and i advise the authors to insert some vewer works, critically explaining them in the text.
All new works added [22, 25, 26, 27, 34, 40, 46, 47, 48, 49,
11. About treatment chances:
-D'Attilio M, Rodolfino D, Filippakos A, Saccucci M, Festa F, Tripodi D Second class resolver: a retrospective analysis Eur J Paedriatic Dent 2014; 15(1):78-82
-Baldini A, Nota A, Santariello C, Assi V, Ballanti F, Gatto R, Cozza P Sagittal dentoskeletal modifications associated with different activation protocols of rapid maxillary expansion Eur J Paedriatic Dent 2018;19(2):151-155
Lines 310-311
With respect in the name of all authors.
Author Response File: Author Response.docx
