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Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolated from War-Injured Patients from the Eastern Ukraine

1
Institute for Medical Microbiology, Immunology, and Hygiene, University of Cologne, 50935 Cologne, Germany
2
German Centre for Infection Research (DZIF), partner site Bonn-Cologne, 50935 Cologne, Germany
3
Department of Microbiology and Hospital Hygiene, Bundeswehr Central Hospital Koblenz, 56070 Koblenz, Germany
4
Institute for Medical Microbiology, Virology and Hygiene, University Medicine Rostock, 18057 Rostock, Germany
5
Department of Microbiology and Hospital Hygiene, Bundeswehr Hospital Hamburg, 20359 Hamburg, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Antibiotics 2020, 9(9), 579; https://doi.org/10.3390/antibiotics9090579
Received: 19 August 2020 / Revised: 1 September 2020 / Accepted: 3 September 2020 / Published: 5 September 2020
(This article belongs to the Special Issue Antibiotic Resistance Genes: Spread and Evolution)
Recently, a total of 32 carbapenem- and fluoroquinolone-resistant Acinetobacter baumannii (Ab) isolates was isolated from war-injured patients who were treated at German Bundeswehr Hospitals, and preliminarily typed by “DiversiLab” repetitive elements sequence-based (rep-) PCR. Core genome-based sequence typing was also used to provide more detailed epidemiological information. From the clusters observed by rep-PCR, selected Ab strains were subjected to Next Generation Sequencing (NGS) in order to compare them with international outbreak-associated Ab strains and to identify MLST (multi-locus sequence type) lineages, as well as to identify known resistance genes. Accordingly, NGS indicated higher diversity than rep-PCR, but also confirmed likely transmission events. The identified acquired carbapenem-resistant genes comprised blaOXA-23, blaOXA-72 and blaGES-12, as well as various other intrinsic and acquired resistance-associated genetic elements. All isolates clustered with the previously identified international clonal lineages IC1, IC2, IC6 and IC7, with corresponding Pasteur sequence types ST1, ST2, ST78 and ST25, respectively. In conclusion, the assessment confirmed a broad spectrum of resistance-associated genes in Ab isolated from war-injured patients from the Eastern Ukraine, and provided the first insights into locally abundant clonal lineages. View Full-Text
Keywords: Acinetobacter baumannii complex; war injury; Ukraine; epidemiology; sequence typing; carbapenem resistance; resistance gene; crisis zone Acinetobacter baumannii complex; war injury; Ukraine; epidemiology; sequence typing; carbapenem resistance; resistance gene; crisis zone
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MDPI and ACS Style

Higgins, P.G.; Hagen, R.M.; Podbielski, A.; Frickmann, H.; Warnke, P. Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolated from War-Injured Patients from the Eastern Ukraine. Antibiotics 2020, 9, 579. https://doi.org/10.3390/antibiotics9090579

AMA Style

Higgins PG, Hagen RM, Podbielski A, Frickmann H, Warnke P. Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolated from War-Injured Patients from the Eastern Ukraine. Antibiotics. 2020; 9(9):579. https://doi.org/10.3390/antibiotics9090579

Chicago/Turabian Style

Higgins, Paul G.; Hagen, Ralf M.; Podbielski, Andreas; Frickmann, Hagen; Warnke, Philipp. 2020. "Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolated from War-Injured Patients from the Eastern Ukraine" Antibiotics 9, no. 9: 579. https://doi.org/10.3390/antibiotics9090579

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