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Antibiotics
Volume 10
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10.3390/antibiotics10060670
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Article

First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing E. coli Isolates from Diarrheic Poultry in Tunisia

by
Ahlem Jouini
1,*,
Amira Klibi
1,
Imen Elarbi
1,
Meriem Ben Chaabene
1,
Safa Hamrouni
1,
Oussema Souiai
2,
Mariem Hanachi
2,3,
Abdeljelil Ghram
1 and
Abderrazak Maaroufi
1
1
Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis 2092, Tunisia
2
Laboratory of Bioinformatics, Biomathematics and Biostatistics-LR16IPT09, Institute Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 2092, Tunisia
3
Faculty of Sciences of Bizerte, University of Carthage, Jarzouna-Bizerte 7021, Tunisia
*
Author to whom correspondence should be addressed.
Antibiotics 2021, 10(6), 670; https://doi.org/10.3390/antibiotics10060670
Submission received: 2 March 2021 / Revised: 27 April 2021 / Accepted: 29 April 2021 / Published: 4 June 2021
(This article belongs to the Special Issue Global Antimicrobial Resistance: What Are the Current Emerging Threats?)
Versions Notes

Abstract

Circulation of a multi-resistance clone of bacteria associated with genetic elements in diseased animals constitutes a global public health problem. Our study focused on the characterization of the support of ESBL in cefotaxime resistant E. coli (CTXR) isolates recovered from poultry with diarrhea, analysis of their clonal lineage, and virulence-associated genes. The study was carried out on 130 samples of chickens with diarrhea, collected in 2015 from poultry farms in Tunisia. Isolates of 20 CTXR E. coli strains were identified as ESBL and AmpC β- lactamase producers. The following β-lactamase genes (number of isolates) were detected: blaCTX-M-15+ blaOXA1 (4), blaCTX-M-15 + blaOXA1 + blaTEM-1b (2), blaCTX-M-1 + blaTEM-1b (9), blaCTX-M-1 (2), blaCMY2 + blaTEM-1b (3). Six E. coli harboring blaCTXM-15 were allocated to ST131-B2-O25b-; six and three blaCTX-M-1 were grouped in ST155, ST10, and ST58, respectively, related to the phylogroup D and A. The qnrB gene, the variant aac(6′)-Ib-cr, and the class 1 integrons with different gene cassettes, were detected amongst our 20 isolated strains, which were classified as ExPEC and aEPEC. Our findings highlighted the emergence of the human pandemic ST131-CTX-M-15-O25-B2 clone and the high risk of such clonal lineage strains in diarrheic poultry, in Tunisia, which could constitute a risk of their transfer to healthy animals and humans.
Keywords: ESBL; ST131; clonal lineages; pAmpC; diarrheic poultry; pathogenic bacteria ESBL; ST131; clonal lineages; pAmpC; diarrheic poultry; pathogenic bacteria

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MDPI and ACS Style

Jouini, A.; Klibi, A.; Elarbi, I.; Chaabene, M.B.; Hamrouni, S.; Souiai, O.; Hanachi, M.; Ghram, A.; Maaroufi, A. First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing E. coli Isolates from Diarrheic Poultry in Tunisia. Antibiotics 2021, 10, 670. https://doi.org/10.3390/antibiotics10060670

AMA Style

Jouini A, Klibi A, Elarbi I, Chaabene MB, Hamrouni S, Souiai O, Hanachi M, Ghram A, Maaroufi A. First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing E. coli Isolates from Diarrheic Poultry in Tunisia. Antibiotics. 2021; 10(6):670. https://doi.org/10.3390/antibiotics10060670

Chicago/Turabian Style

Jouini, Ahlem, Amira Klibi, Imen Elarbi, Meriem Ben Chaabene, Safa Hamrouni, Oussema Souiai, Mariem Hanachi, Abdeljelil Ghram, and Abderrazak Maaroufi. 2021. "First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing E. coli Isolates from Diarrheic Poultry in Tunisia" Antibiotics 10, no. 6: 670. https://doi.org/10.3390/antibiotics10060670

APA Style

Jouini, A., Klibi, A., Elarbi, I., Chaabene, M. B., Hamrouni, S., Souiai, O., Hanachi, M., Ghram, A., & Maaroufi, A. (2021). First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing E. coli Isolates from Diarrheic Poultry in Tunisia. Antibiotics, 10(6), 670. https://doi.org/10.3390/antibiotics10060670

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